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1. (Article ID: 5844)
Sigida EN, Shashkov AS, Zdorovenko EL, Konnova SA, Fedonenko YP
Structure of the O-specific polysaccharide from Azospirillum formosense CC-Nfb-7(T)
Carbohydrate Research 494 (2020)
108060
The lipopolysaccharide was obtained from the cells of Azospirillum formosense CC-Nfb-7(T), a diazotrophic bacterium isolated from agricultural soil. The O-specific polysaccharide (OPS) was released by mild acid hydrolysis of the lipopolysaccharide and was studied by sugar analysis along with (1)H and (13)C NMR spectroscopy, including (1)H,(1)H COSY, TOCSY, ROESY, (1)H,(13)C HSQC, and HMBC experiments, and Smith degradation. The following structure of partially methylated OPS composed of trisaccharide repeating units was established.
Lipopolysaccharide, structure, O-specific polysaccharide, biochemistry, physiology, 3-O-Methyl-DS-mannose, Azospirillum formosense
NCBI PubMed ID: 32569849Publication DOI: 10.1016/j.carres.2020.108060Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: E.N. Sigida
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Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov, 410049, Russia, Saratov State University, 83 Ulitsa Astrakhanskaya, Saratov, 410012, Russia
Methods: 13C NMR, 1H NMR, NMR-2D, SDS-PAGE, sugar analysis, GLC, mild acid hydrolysis, Smith degradation, GPC
The publication contains the following compound(s):
- Compound ID: 14935
Structure type: polymer chemical repeating unit
Compound class: O-polysaccharide
Reference(s) to other database(s): GTC:G29218PE
- Compound ID: 14936
Structure type: oligomer
Compound class: O-polysaccharide
Reference(s) to other database(s): GTC:G01931AE
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