The paper reports the structural study of the lipopolysaccharide from the type strain of the soil nitrogen-fixing bacterium Azospirillum rugosum isolated from oil-polluted soils. Based on the chemical analysis and 1D- and 2D 1H and 13C NMR spectroscopy data it has been demonstrated that the O-specific polysaccharide consists of the two types of repeating units identical to those previously described for the O-specific polysaccharide of the Azospirillum brasilense strain Jm125A2. Structural analysis of the lipid A using gas-liquid chromatography (GLC) and MALDI mass spectrometry revealed the presence of penta-, tetra-, and triacylated species. The primary fatty acids in the lipid A are the N-linked 16:0(3-OH) and O-linked 14:0(3-OH). Microheterogeneity within each species arises from the presence of different secondary fatty acids (16:0, 18:1, or 19:0) acylating the 16:0(3-OH) acid of the distal GlcN ring.
Publication DOI: 10.1134/S1068162020010112Journal NLM ID: 9420101Publisher: Springer Science and Business Media
Correspondence: si_elena
mail.ru
Institutions: Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 410049, Saratov, Russia, Elyakov Pacific Institute of Bioorganic Chemistry, Far-Eastern Branch, Russian Academy of Sciences, 690022, Vladivostok, Russia, Chernyshevskii Saratov National Research State University, 410012, Saratov, Saratov oblast, Russia
Methods: 13C NMR, 1H NMR, methylation, gel filtration, NMR-2D, de-O-acylation, sugar analysis, acid hydrolysis, GLC, GC, MS/MS, MALDI-TOF MS, composition analysis
Found