The bacteria from Ochrobactrum genus are commonly found in the soil and association with the roots of plants. The O-polysaccharide and the glucan were isolated from the Ochrobactrum rhizosphaerae PR17(T) strain. Purified polysaccharides were analysed using chemical methods and NMR spectroscopy. Sugar and absolute configuration assignment combined with NMR data revealed the chemical structure of the repeating unit of the O- [formula: see text].

NMR, structure, O-polysaccharide, glucan, Ochrobactrum rhizosphaerae PR17(T)

NCBI PubMed ID: 32889436
Publication DOI: 10.1016/j.carres.2020.108136
Journal NLM ID: 0043535
Publisher: Elsevier
Correspondence: zbigniew.kaczynskiug.edu.pl
Institutions: Faculty of Chemistry, University of Gdansk, Gdansk, Poland, Laboratory of Biological Plant Protection, Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of Gdansk, University of Gdansk, Gdansk, Poland
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, acid hydrolysis, GLC, composition analysis, acetylation, SEC, reduction with NaBH4
 

• Compound ID: 431
  

  -3)-a-D-GlcpNAc-(1-2)-a-L-Rhap-(1-

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  Structure type: polymer chemical repeating unit
  Compound class: O-polysaccharide
  Reference(s) to other database(s): GTC:G23685LC, GlycomeDB:26574
  
   
  
  Ochrobactrum anthropi LMG 3331
  CSDB ID 7596 (all data & tools)
• Compound ID: 14965
  

  /Variants 0/-+ | -3)-b-D-GalpNAc-(1-3)-a-D-Fucp-(1- /Variants 0/ is: 10%Pyr-(2-6:2-4)- OR (exclusively) 10%Me-4)-

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  Structure type: polymer chemical repeating unit
  Compound class: O-polysaccharide
  
   
  
  Ochrobactrum cytisi ESC1T
  CSDB ID 7594 (all data & tools)
• Compound ID: 14938
  

  -6)-a-D-Glcp-(1-4)-b-D-ManpNAcA-(1-4)-b-D-GlcpNAc3Ac6(50%)Ac-(1-

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  Structure type: polymer chemical repeating unit
  Compound class: O-polysaccharide
  Reference(s) to other database(s): GTC:G83227MO
  
   
  
  Ochrobactrum cytisi IPA7.2
  CSDB ID 7595 (all data & tools)
• Compound ID: 6655
  

  Cyclic -2)-b-D-Glcp-(1-

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  Structure type: cyclic polymer repeating unit ; n=17-25
  Trivial name: cyclosophoran, cyclic β-1,2-glucan, cyclic b-glucan
  Compound class: EPS, glucan, cyclic b-glucan
  Reference(s) to other database(s): GTC:G21553SY, GlycomeDB:25048
  
   
  
  Ochrobactrum rhizosphaerae PR17(T)
  CSDB ID 7593 (all data & tools)
• Compound ID: 14964
  

  a-D-Fucp-(1-6)-+ | -4)-b-D-GalpNAc-(1-3)-a-D-Fucp-(1-

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  Structure type: polymer chemical repeating unit
  Compound class: O-polysaccharide
  Reference(s) to other database(s): GTC:G07468HH
  
   
  
  Ochrobactrum rhizosphaerae PR17(T)
  CSDB ID 32210 (all data & tools)