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1. (Article ID: 5858)
 
Velichko NS, Kokoulin MS, Sigida EN, Kuchur PD, Komissarov AS, Kovtunov EA, Fedonenko YP
Structural and genetic characterization of the colitose-containing O-specific polysaccharide from the lipopolysaccharide of Herbaspirillum frisingense GSF30(T)
International Journal of Biological Macromolecules 161 (2020) 891-897
 

The lipopolysaccharide (LPS) of Herbaspirillum frisingense GSF30(T) (HfGSF30), a non-pathogenic diazotrophic endobiont, was isolated by phenol-water extraction from bacterial cells and was characterized by chemical analyses and SDS PAGE. The O-specific polysaccharide (OPS, O-antigen), obtained by mild acid hydrolysis of the LPS, was examined by sugar and methylation analysis, along with (1)H and (13)C NMR spectroscopy, including 2D (1)H,(1)H COSY, (1)H,(1)H TOCSY, (1)H,(1)H ROESY, (1)H,(13)C HSQC, and (1)H,(13)C HMBC experiments. The OPS was found to consist of branched tetrasaccharide repeating units of the following structure: [Formula: see text] This structure is unique among the known bacterial polysaccharide structures. Analysis of the HfGSF30 genome showed that it contained a set of sequentially arranged operons (presumably a cluster of genes) associated with the O-antigen. Amino acid sequence analysis using the BLAST program demonstrated the specificity of this putative cluster for Herbaspirillum spp. The genes responsible for the biosynthesis of the OPS of HfGSF30 were dispersed in the genome, constituting small operons. A putative O-antigen gene cluster of HfGSF30 was identified and found to be consistent with the OPS structure.

O antigen, O-specific polysaccharide, bacterial polysaccharide structure, colitose, Herbaspirillum frisingense, O-antigen genes

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2. (Article ID: 10877)
 
Frias J, Hedley CL, Price KR, Fenwick GR, Vidal-Valverde C
Improved methods of oligosaccharide analysis for genetic studies of legume seeds
Journal of Liquid Chromatography 17 (1994) 2469-2483
 

The analysis of low molecular weight carbohydrates in single seed of lentils has been carried out using two different high performance liquid chromatography (HPLC) methods. One used a reversed-phase column coupled to a refractive index detector (HPLC-RI), while the other utilised an anion-exchange phase column coupled to a triple-pulsed amperometric detector (HPAC-PAD). The latter was found to he more sensitive and could be used for the analysis of very small samples, hence allowing parts of a seed to be analysed and then grown and used for genetic studies.

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