Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
The structure was elucidated in this paperNCBI PubMed ID: 11448680Publication DOI: 10.1016/S0008-6215(01)00123-9Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: G. Widmalm <gw
organ.su.se>
Institutions: Arrhenius Laboratory, Department of Organic Chemistry, Stockholm University, Stockholm, Sweden, Karolinska Institute, Department of Microbiology, Pathology and Immunology, Division of Clinical Bacteriology, Huddinge University Hospital, Stockholm, Sweden
The structure of the O-antigen polysaccharide (PS) from Escherichia coli O77 has been determined. Sugar and methylation analysis together with 1H and 13C NMR spectroscopy were the main methods used. The PS is composed of tetrasaccharide repeating units with the following structure [see text]
Lipopolysaccharide, NMR, Escherichia coli
Structure type: suggested polymer biological repeating unit
Location inside paper: abstract
Compound class: O-polysaccharide, core oligosaccharide, O-antigen
Contained glycoepitopes: IEDB_130701,IEDB_136104,IEDB_137340,IEDB_137485,IEDB_140116,IEDB_141807,IEDB_141830,IEDB_143632,IEDB_144983,IEDB_151531,IEDB_152206,IEDB_983930,SB_136,SB_196,SB_44,SB_67,SB_72
Methods: methylation, NMR-2D, NMR, sugar analysis
Biological activity: serological data
Related record ID(s): 6049, 11268, 20665, 21421, 25629, 30340
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G87308MU, GlycomeDB:
2505
Show glycosyltransferases
NMR conditions: in D2O at 308 K
[as TSV]
13C NMR data:
Linkage Residue C1 C2 C3 C4 C5 C6
3,2,2 aDManp 103.0 70.4 71.2 66.4 72.0 65.4
3,2 aDManp 99.9 78.8 70.4 67.0 73.0 61.2
3 bDManp 100.4 76.3 74.3 67.3 77.3 61.4
2 Ac 174.2 22.6
aDGlcpN 97.6 53.5 80.6 68.8 72.3 61.1
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,2,2 aDManp 5.05 4.12 3.86 3.97 3.82 3.55-4.11
3,2 aDManp 5.32 4.08 4.03 3.77 3.99 3.84
3 bDManp 4.76 3.97 3.72 3.65 3.42 3.76-3.96
2 Ac - 2.06
aDGlcpN 4.88 4.08 3.95 3.56 3.76 3.80-3.88
1H/13C HSQC data:
Linkage Residue C1/H1 C2/H2 C3/H3 C4/H4 C5/H5 C6/H6
3,2,2 aDManp 103.0/5.05 70.4/4.12 71.2/3.86 66.4/3.97 72.0/3.82 65.4/3.55-4.11
3,2 aDManp 99.9/5.32 78.8/4.08 70.4/4.03 67.0/3.77 73.0/3.99 61.2/3.84
3 bDManp 100.4/4.76 76.3/3.97 74.3/3.72 67.3/3.65 77.3/3.42 61.4/3.76-3.96
2 Ac 22.6/2.06
aDGlcpN 97.6/4.88 53.5/4.08 80.6/3.95 68.8/3.56 72.3/3.76 61.1/3.80-3.88
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,2,2 | aDManp | 5.05 | 4.12 | 3.86 | 3.97 | 3.82 | 3.55
4.11 |
| 3,2 | aDManp | 5.32 | 4.08 | 4.03 | 3.77 | 3.99 | 3.84 |
| 3 | bDManp | 4.76 | 3.97 | 3.72 | 3.65 | 3.42 | 3.76
3.96 |
| 2 | Ac |
| 2.06 | |
| | aDGlcpN | 4.88 | 4.08 | 3.95 | 3.56 | 3.76 | 3.80
3.88 |
|
13C NMR data:
| Linkage | Residue | C1 | C2 | C3 | C4 | C5 | C6 |
|---|
| 3,2,2 | aDManp | 103.0 | 70.4 | 71.2 | 66.4 | 72.0 | 65.4 |
| 3,2 | aDManp | 99.9 | 78.8 | 70.4 | 67.0 | 73.0 | 61.2 |
| 3 | bDManp | 100.4 | 76.3 | 74.3 | 67.3 | 77.3 | 61.4 |
| 2 | Ac | 174.2 | 22.6 | |
| | aDGlcpN | 97.6 | 53.5 | 80.6 | 68.8 | 72.3 | 61.1 |
|
There is only one chemically distinct structure:
Found 
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