Found 12 structures.
Displayed structures from 1 to 12
Expand all compounds
Collapse all compounds
Show all as text (SweetDB notation)
Show all graphically (SNFG notation)
1. Compound ID: 5962
|
b-D-GalpNAc-(1-3)-b-D-GalpNAc-(1-3)-a-D-Galp-(1-4)-b-D-Galp-(1-4)-b-D-Glcp-(1-?)-CER-(?--/Ceramide/ |
Show graphically |
Structure type: oligomer
Aglycon: Ceramide
Trivial name: IV3GalNAcb-Gb4-Cer, para-Forssman glycolipid
Compound class: glycosphingolipid
Contained glycoepitopes: IEDB_130648,IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137339,IEDB_137472,IEDB_137473,IEDB_1391964,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_146664,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_18,SB_187,SB_19,SB_192,SB_195,SB_21,SB_27,SB_3,SB_31,SB_5,SB_6,SB_62,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 2655
McConville MJ, Blackwell JM "Developmental changes in the glycosylated phosphatidylinositols of Leishmania donovani. Characterization of the promastigote and amastigote glycolipids" -
Journal of Biological Chemistry 266 (1991) 15170-15179
In addition to utilizing glycosylated phosphatidylinositols (GPIs) as anchors for surface proteins, protozoan parasites of the genus Leishmania synthesize two novel classes of GPI: the polydisperse lipophosphoglycans (LPGs) and a family of low molecular weight glycoinositol phospholipids (GIPLs). We now show that LPG is expressed in high copy number (6 x 10(6) molecules/cell) in the promastigote (insect) stage of L. donovani but not in the amastigote stage, which infects mammalian macrophages. Detection of these molecules was by gas chromatography-mass spectrometric analyses and by a sensitive radiolabeling procedure. In contrast, a novel family of GIPLs was present in high copy number (approximately 10(7) molecules/cell) in both promastigote and amastigote stages of L. donovani. These glycolipids were purified and analyzed by gas chromatography-mass spectrometry, methylation analysis, and by chemical and enzymatic sequencing after deamination and NaB3H4 reduction. Promastigotes contained three major GIPLs species with the following generalized structure [formula: see text] where R = H for isoM2, Man α1- for isoM3 or Man α1-2Man α1- for isoM4. Amastigotes contained two major GIPL species that lacked the α1-3-linked mannose branch and had the linear structures Man α1-6Man α1-4GlcN (M2) and Man α1-2Man α1-6Man α1-4GlcN (M3) linked to alkylacyl-PI. The 1-O-alkyl-2-acyl-PI moieties of all these species contained predominantly C18:0 alkyl chains and C16:0 or C18:0 fatty acids. Amastigotes contained, in addition, a GalNAc β1-3 terminating glycosphingolipid with homology to the mammalian para Forssman glycolipid. This glycolipid appeared to be a constituent of the parasite membrane but was not metabolically labeled with [3H]glucose, suggesting that it was acquired from host cells. These results suggest that LPG may not be required for amastigote survival in the mammalian host and that the GIPLs are likely to be major components on the surface membrane in both stages.
NCBI PubMed ID: 1831200Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Institutions: Department of Biochemistry, University of Dundee, United Kingdom
Expand this compound
Collapse this compound
2. Compound ID: 5963
|
a-D-Galp-(1-4)-b-D-Galp-(1-4)-b-D-Glcp-(1-?)-CER-(?--/Ceramide/ |
Show graphically |
Structure type: oligomer
Aglycon: Ceramide
Trivial name: Gb3-Cer
Compound class: glycosphingolipid
Contained glycoepitopes: IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137339,IEDB_137472,IEDB_1391964,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_146664,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_27,SB_3,SB_31,SB_5,SB_6,SB_62,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 2656
John CM, Griffiss JM, Apicella MA, Mandrell RE, Gibson BW "The structural basis for pyocin resistance in Neisseria gonorrhoeae lipooligosaccharides" -
Journal of Biological Chemistry 266 (1991) 19303-19311
Pyocin resistance in a strain of Neisseria gonorrhoeae has been found to be associated with structural differences in the oligosaccharide moieties of the gonococcal outer membrane lipooligosaccharides (LOS). N. gonorrhoeae strain 1291 had been treated with several pyocins, usually lethal bacteriocins produced by Pseudomonas aeruginosa, and a series of surviving mutants were selected. The LOS of these pyocin-resistant mutants had altered electrophoretic mobilities in sodium dodecyl sulfate-polyacrylamide gels (Dudas, K. C., and Apicella, M. A. (1988) Infect. Immun. 56, 499-504). Structural analyses of the oligosaccharide portions of the wild-type (1291 wt) and five pyocin-resistant strains (1291a-e) by liquid secondary ion mass spectrometry, tandem mass spectrometry, and methylation analysis revealed that four of the mutant strains make oligosaccharides that differ from the wild-type LOS by successive saccharide deletions (1291a,c-e) and, in the oligosaccharide of 1291b, by the addition of a terminal Gal to the 1291c structure. The composition, sequence, and linkages of the terminal tetrasaccharide of the wild-type LOS are the same as the lacto-N-neotetraose terminus of the human paragloboside (Gal β1→4 GlcNAc β1→3 Gal β1→4 Glc-ceramide), and both glycolipids bound the same monoclonal antibodies O6B4/3F11 that recognize this terminal epitope. None of the pyocin-resistant mutants bound this antibody. The 1291b LOS bound a monoclonal antibody that is specific for Gal α1→4 Gal β1→4 Glc-ceramide (Pk glycosphingolipid) and shared a common composition, sequence, and linkages with this latter glycosphingolipid. Organisms that bound the anti-Pk monoclone occurred at the rate of approximately 1/750 among the wild-type parent strain. This structural information supports the conclusion that treatment with pyocin selects for mutants with truncated LOS structures and suggests that the oligosaccharides contained in the LOS of the wild-type strain and 1291b mimic those of human glycosphingolipids.
NCBI PubMed ID: 1918047Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Institutions: Department of Pharmaceutical Chemistry, University of California, San Francisco, CA, USA
- Article ID: 2664
Straus AH, Levery SB, Jasiulionis MG, Salyan MEK, Steele SJ, Travassos LR, Hakomori SI, Takahashi HK "Stage-specific glycosphingolipids form amastigote forms of Leishmania (L.) amazonensis. Immunogenicity and role in parasite binding and invasion of macrophages" -
Journal of Biological Chemistry 268 (1993) 13723-13730
Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Methods: 1H NMR, FAB-MS, GC-MS
Expand this compound
Collapse this compound
3. Compound ID: 5973
|
b-D-Galp-(1-3)-a-D-Galp-(1-4)-b-D-Galp-(1-4)-b-D-Glcp-(1-?)-CER-(?--/Ceramide/ |
Show graphically |
Structure type: oligomer
Aglycon: Ceramide
Compound class: glycosphingolipid
Contained glycoepitopes: IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137339,IEDB_137472,IEDB_1391964,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_146664,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_27,SB_3,SB_31,SB_5,SB_6,SB_62,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 2664
Straus AH, Levery SB, Jasiulionis MG, Salyan MEK, Steele SJ, Travassos LR, Hakomori SI, Takahashi HK "Stage-specific glycosphingolipids form amastigote forms of Leishmania (L.) amazonensis. Immunogenicity and role in parasite binding and invasion of macrophages" -
Journal of Biological Chemistry 268 (1993) 13723-13730
Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Methods: 1H NMR, FAB-MS, GC-MS
Expand this compound
Collapse this compound
4. Compound ID: 5975
|
b-D-GalpNAc-(1-3)-a-D-Galp-(1-4)-b-D-Galp-(1-4)-b-D-Glcp-(1-?)-CER-(?--/Ceramide/ |
Show graphically |
Structure type: oligomer
Aglycon: Ceramide
Trivial name: Gb4-Cer, Globoside
Compound class: glycosphingolipid
Contained glycoepitopes: IEDB_130648,IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137339,IEDB_137472,IEDB_137473,IEDB_1391964,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_146664,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_18,SB_187,SB_192,SB_195,SB_21,SB_27,SB_3,SB_31,SB_5,SB_6,SB_62,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 2664
Straus AH, Levery SB, Jasiulionis MG, Salyan MEK, Steele SJ, Travassos LR, Hakomori SI, Takahashi HK "Stage-specific glycosphingolipids form amastigote forms of Leishmania (L.) amazonensis. Immunogenicity and role in parasite binding and invasion of macrophages" -
Journal of Biological Chemistry 268 (1993) 13723-13730
Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Methods: 1H NMR, FAB-MS, GC-MS
Expand this compound
Collapse this compound
5. Compound ID: 10269
|
b-GalNAc-(1-3)-a-Gal-(1-4)-b-Gal-(1-4)-b-Glc-(1-?)-CER-(?--/trimethylsylilethyl (instead of natural Cer)/ |
Show graphically |
Structure type: oligomer
Aglycon: trimethylsylilethyl (instead of natural Cer)
Trivial name: GbO4
Contained glycoepitopes: IEDB_130648,IEDB_130651,IEDB_136044,IEDB_136095,IEDB_136906,IEDB_137339,IEDB_137472,IEDB_137473,IEDB_1391964,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_146664,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_18,SB_187,SB_192,SB_195,SB_21,SB_27,SB_3,SB_31,SB_5,SB_6,SB_62,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 4255
Dodson KW, Pinkner JS, Rose T, Magnusson G, Hultgren SJ, Waksman G "Structural basis of the interaction of the pyelonephritic E. coli adhesin to its human kidney receptor" -
Cell 105(6) (2001) 733-743
PapG is the adhesin at the tip of the P pilus that mediates attachment of uropathogenic Escherichia coli to the uroepithelium of the human kidney. The human specific allele of PapG binds to globoside (GbO4), which consists of the tetrasaccharide GalNAc β 1-3Gal α 1-4Gal β 1-4Glc linked to ceramide. Here, we present the crystal structures of a binary complex of the PapG receptor binding domain bound to GbO4 as well as the unbound form of the adhesin. The biological importance of each of the residues involved in binding was investigated by site-directed mutagenesis. These studies provide a molecular snapshot of a host-pathogen interaction that determines the tropism of uropathogenic E. coli for the human kidney and is critical to the pathogenesis of pyelonephritis.
chemistry, Bacterial, metabolism, microbiology, pathogenicity, molecular, X-ray, Escherichia coli, Female, Molecular Sequence Data, proteins, mutagenesis, protein structure, models, amino acid sequence, Sequence Alignment, kidney, Fimbriae, Protein Binding, Crystallography, Humans, Adhesins, Binding Sites, Escherichia coli Infections, Crystallization, Fimbriae Proteins, Globosides, Protein Conformation, Tertiary, Pyelonephritis, Urothelium
NCBI PubMed ID: 11440716Journal NLM ID: 0413066Publisher: Cambridge, MA: Cell Press
Institutions: Department of Molecular Microbiology, Washington University School of Medicine, Saint Louis, MO 63110, USA
Methods: MAD
Expand this compound
Collapse this compound
6. Compound ID: 24346
Structure type: oligomer
Compound class: glycolipid, glycosphingolipid
Contained glycoepitopes: IEDB_115015,IEDB_130651,IEDB_136044,IEDB_136045,IEDB_136095,IEDB_136105,IEDB_136906,IEDB_137339,IEDB_137472,IEDB_1391964,IEDB_1394181,IEDB_141794,IEDB_141798,IEDB_142487,IEDB_142488,IEDB_142489,IEDB_144562,IEDB_144987,IEDB_144998,IEDB_146664,IEDB_149135,IEDB_151528,IEDB_152214,IEDB_152217,IEDB_174333,IEDB_190606,IEDB_221845,IEDB_225177,IEDB_423106,IEDB_742247,IEDB_885823,IEDB_983931,SB_143,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_27,SB_3,SB_31,SB_5,SB_6,SB_62,SB_7,SB_86,SB_88
The structure is contained in the following publication(s):
- Article ID: 9945
Rezanka T, Mareš P "Preparative separation of sphingolipids and of individual molecular species by high-performance liquid chromatography and their identification by gas chromatography-mass spectrometry" -
Journal of Chromatography 509 (1990) 333-346
Six fractions containing tri- to pentaglycosylceramides were isolated from the green, fresh water alga Chlorella kessleri, grown heterotrophically, by using preparative high-performance liquid chromatography (HPLC). Up to twelve fractions were obtained by further reversed-phase HPLC of each glycosylceramide. The use of a polar capillary column with Supelcowax 10 as the stationary phase allowed an excellent separation of the individual molecular species of ceramides, even though the separation did not occur when the ceramides differed only in the position of the amide bond. The individual molecular species (even if present in mixtures) were identified by gas chromatography-chemical ionization mass spectrometry. The evidence for a complete structure was obtained by enzyme splitting with α- and β-galactosidases (the sequence of monosaccharides) and by negative ionization fast atom bombardment mass spectrometry. More than 400 molecular species of glycosylceramides were identified.
NCBI PubMed ID: 2211899Publication DOI: 10.1016/S0021-9673(01)93091-2Journal NLM ID: 0427043Publisher: Amsterdam: Elsevier
Institutions: Institute of Microbiology, Czechoslovak Academy of Sciences, Prague, Czechoslovakia, Lipid Laboratory, Faculty of Medicine, Charles University, Prague, Czechoslovakia
Methods: GC-MS, acid hydrolysis, HPLC, alkaline hydrolysis, enzymatic digestion, RP-HPLC, NI-FAB-MS
Expand this compound
Collapse this compound
7. Compound ID: 24347
Structure type: oligomer
Compound class: glycolipid, glycosphingolipid
Contained glycoepitopes: IEDB_130651,IEDB_136044,IEDB_136095,IEDB_136105,IEDB_136906,IEDB_137339,IEDB_137472,IEDB_1391964,IEDB_1394181,IEDB_141794,IEDB_141798,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_221845,IEDB_225177,IEDB_423106,IEDB_742247,IEDB_885823,IEDB_983931,SB_143,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_27,SB_3,SB_31,SB_5,SB_6,SB_62,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 9945
Rezanka T, Mareš P "Preparative separation of sphingolipids and of individual molecular species by high-performance liquid chromatography and their identification by gas chromatography-mass spectrometry" -
Journal of Chromatography 509 (1990) 333-346
Six fractions containing tri- to pentaglycosylceramides were isolated from the green, fresh water alga Chlorella kessleri, grown heterotrophically, by using preparative high-performance liquid chromatography (HPLC). Up to twelve fractions were obtained by further reversed-phase HPLC of each glycosylceramide. The use of a polar capillary column with Supelcowax 10 as the stationary phase allowed an excellent separation of the individual molecular species of ceramides, even though the separation did not occur when the ceramides differed only in the position of the amide bond. The individual molecular species (even if present in mixtures) were identified by gas chromatography-chemical ionization mass spectrometry. The evidence for a complete structure was obtained by enzyme splitting with α- and β-galactosidases (the sequence of monosaccharides) and by negative ionization fast atom bombardment mass spectrometry. More than 400 molecular species of glycosylceramides were identified.
NCBI PubMed ID: 2211899Publication DOI: 10.1016/S0021-9673(01)93091-2Journal NLM ID: 0427043Publisher: Amsterdam: Elsevier
Institutions: Institute of Microbiology, Czechoslovak Academy of Sciences, Prague, Czechoslovakia, Lipid Laboratory, Faculty of Medicine, Charles University, Prague, Czechoslovakia
Methods: GC-MS, acid hydrolysis, HPLC, alkaline hydrolysis, enzymatic digestion, RP-HPLC, NI-FAB-MS
Expand this compound
Collapse this compound
8. Compound ID: 24348
Structure type: oligomer
Compound class: glycolipid, glycosphingolipid
Contained glycoepitopes: IEDB_115015,IEDB_130651,IEDB_136044,IEDB_136045,IEDB_136095,IEDB_136906,IEDB_137339,IEDB_137472,IEDB_1391964,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_142489,IEDB_144562,IEDB_144987,IEDB_144998,IEDB_146664,IEDB_149135,IEDB_151528,IEDB_152214,IEDB_152217,IEDB_174333,IEDB_190606,IEDB_221845,IEDB_423106,IEDB_742247,IEDB_983931,SB_143,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_27,SB_3,SB_31,SB_5,SB_6,SB_62,SB_7,SB_86,SB_88
The structure is contained in the following publication(s):
- Article ID: 9945
Rezanka T, Mareš P "Preparative separation of sphingolipids and of individual molecular species by high-performance liquid chromatography and their identification by gas chromatography-mass spectrometry" -
Journal of Chromatography 509 (1990) 333-346
Six fractions containing tri- to pentaglycosylceramides were isolated from the green, fresh water alga Chlorella kessleri, grown heterotrophically, by using preparative high-performance liquid chromatography (HPLC). Up to twelve fractions were obtained by further reversed-phase HPLC of each glycosylceramide. The use of a polar capillary column with Supelcowax 10 as the stationary phase allowed an excellent separation of the individual molecular species of ceramides, even though the separation did not occur when the ceramides differed only in the position of the amide bond. The individual molecular species (even if present in mixtures) were identified by gas chromatography-chemical ionization mass spectrometry. The evidence for a complete structure was obtained by enzyme splitting with α- and β-galactosidases (the sequence of monosaccharides) and by negative ionization fast atom bombardment mass spectrometry. More than 400 molecular species of glycosylceramides were identified.
NCBI PubMed ID: 2211899Publication DOI: 10.1016/S0021-9673(01)93091-2Journal NLM ID: 0427043Publisher: Amsterdam: Elsevier
Institutions: Institute of Microbiology, Czechoslovak Academy of Sciences, Prague, Czechoslovakia, Lipid Laboratory, Faculty of Medicine, Charles University, Prague, Czechoslovakia
Methods: GC-MS, acid hydrolysis, HPLC, alkaline hydrolysis, enzymatic digestion, RP-HPLC, NI-FAB-MS
Expand this compound
Collapse this compound
9. Compound ID: 24349
Structure type: oligomer
Compound class: glycolipid, glycosphingolipid
Contained glycoepitopes: IEDB_115015,IEDB_130651,IEDB_136044,IEDB_136045,IEDB_136095,IEDB_136105,IEDB_136906,IEDB_137339,IEDB_137472,IEDB_1391964,IEDB_1394181,IEDB_141794,IEDB_141798,IEDB_142487,IEDB_142488,IEDB_142489,IEDB_144562,IEDB_144987,IEDB_144998,IEDB_146664,IEDB_149135,IEDB_151528,IEDB_152214,IEDB_152217,IEDB_174333,IEDB_190606,IEDB_221845,IEDB_225177,IEDB_423106,IEDB_742247,IEDB_885823,IEDB_983931,SB_143,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_27,SB_3,SB_31,SB_5,SB_6,SB_62,SB_7,SB_86,SB_88
The structure is contained in the following publication(s):
- Article ID: 9945
Rezanka T, Mareš P "Preparative separation of sphingolipids and of individual molecular species by high-performance liquid chromatography and their identification by gas chromatography-mass spectrometry" -
Journal of Chromatography 509 (1990) 333-346
Six fractions containing tri- to pentaglycosylceramides were isolated from the green, fresh water alga Chlorella kessleri, grown heterotrophically, by using preparative high-performance liquid chromatography (HPLC). Up to twelve fractions were obtained by further reversed-phase HPLC of each glycosylceramide. The use of a polar capillary column with Supelcowax 10 as the stationary phase allowed an excellent separation of the individual molecular species of ceramides, even though the separation did not occur when the ceramides differed only in the position of the amide bond. The individual molecular species (even if present in mixtures) were identified by gas chromatography-chemical ionization mass spectrometry. The evidence for a complete structure was obtained by enzyme splitting with α- and β-galactosidases (the sequence of monosaccharides) and by negative ionization fast atom bombardment mass spectrometry. More than 400 molecular species of glycosylceramides were identified.
NCBI PubMed ID: 2211899Publication DOI: 10.1016/S0021-9673(01)93091-2Journal NLM ID: 0427043Publisher: Amsterdam: Elsevier
Institutions: Institute of Microbiology, Czechoslovak Academy of Sciences, Prague, Czechoslovakia, Lipid Laboratory, Faculty of Medicine, Charles University, Prague, Czechoslovakia
Methods: GC-MS, acid hydrolysis, HPLC, alkaline hydrolysis, enzymatic digestion, RP-HPLC, NI-FAB-MS
Expand this compound
Collapse this compound
10. Compound ID: 24350
Structure type: oligomer
Compound class: glycolipid, glycosphingolipid
Contained glycoepitopes: IEDB_130651,IEDB_136044,IEDB_136095,IEDB_136105,IEDB_136906,IEDB_137339,IEDB_137472,IEDB_1391964,IEDB_1394181,IEDB_141794,IEDB_141798,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_221845,IEDB_225177,IEDB_423106,IEDB_742247,IEDB_885823,IEDB_983931,SB_143,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_27,SB_3,SB_31,SB_5,SB_6,SB_62,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 9945
Rezanka T, Mareš P "Preparative separation of sphingolipids and of individual molecular species by high-performance liquid chromatography and their identification by gas chromatography-mass spectrometry" -
Journal of Chromatography 509 (1990) 333-346
Six fractions containing tri- to pentaglycosylceramides were isolated from the green, fresh water alga Chlorella kessleri, grown heterotrophically, by using preparative high-performance liquid chromatography (HPLC). Up to twelve fractions were obtained by further reversed-phase HPLC of each glycosylceramide. The use of a polar capillary column with Supelcowax 10 as the stationary phase allowed an excellent separation of the individual molecular species of ceramides, even though the separation did not occur when the ceramides differed only in the position of the amide bond. The individual molecular species (even if present in mixtures) were identified by gas chromatography-chemical ionization mass spectrometry. The evidence for a complete structure was obtained by enzyme splitting with α- and β-galactosidases (the sequence of monosaccharides) and by negative ionization fast atom bombardment mass spectrometry. More than 400 molecular species of glycosylceramides were identified.
NCBI PubMed ID: 2211899Publication DOI: 10.1016/S0021-9673(01)93091-2Journal NLM ID: 0427043Publisher: Amsterdam: Elsevier
Institutions: Institute of Microbiology, Czechoslovak Academy of Sciences, Prague, Czechoslovakia, Lipid Laboratory, Faculty of Medicine, Charles University, Prague, Czechoslovakia
Methods: GC-MS, acid hydrolysis, HPLC, alkaline hydrolysis, enzymatic digestion, RP-HPLC, NI-FAB-MS
Expand this compound
Collapse this compound
11. Compound ID: 24351
Structure type: oligomer
Compound class: glycolipid, glycosphingolipid
Contained glycoepitopes: IEDB_130651,IEDB_136044,IEDB_136095,IEDB_136906,IEDB_137339,IEDB_137472,IEDB_1391964,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_144998,IEDB_146664,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_221845,IEDB_423106,IEDB_742247,IEDB_983931,SB_143,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_27,SB_3,SB_31,SB_5,SB_6,SB_62,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 9945
Rezanka T, Mareš P "Preparative separation of sphingolipids and of individual molecular species by high-performance liquid chromatography and their identification by gas chromatography-mass spectrometry" -
Journal of Chromatography 509 (1990) 333-346
Six fractions containing tri- to pentaglycosylceramides were isolated from the green, fresh water alga Chlorella kessleri, grown heterotrophically, by using preparative high-performance liquid chromatography (HPLC). Up to twelve fractions were obtained by further reversed-phase HPLC of each glycosylceramide. The use of a polar capillary column with Supelcowax 10 as the stationary phase allowed an excellent separation of the individual molecular species of ceramides, even though the separation did not occur when the ceramides differed only in the position of the amide bond. The individual molecular species (even if present in mixtures) were identified by gas chromatography-chemical ionization mass spectrometry. The evidence for a complete structure was obtained by enzyme splitting with α- and β-galactosidases (the sequence of monosaccharides) and by negative ionization fast atom bombardment mass spectrometry. More than 400 molecular species of glycosylceramides were identified.
NCBI PubMed ID: 2211899Publication DOI: 10.1016/S0021-9673(01)93091-2Journal NLM ID: 0427043Publisher: Amsterdam: Elsevier
Institutions: Institute of Microbiology, Czechoslovak Academy of Sciences, Prague, Czechoslovakia, Lipid Laboratory, Faculty of Medicine, Charles University, Prague, Czechoslovakia
Methods: GC-MS, acid hydrolysis, HPLC, alkaline hydrolysis, enzymatic digestion, RP-HPLC, NI-FAB-MS
Expand this compound
Collapse this compound
12. Compound ID: 30906
Structure type: oligomer
Compound class: glycolipid, glycosylceramide
Contained glycoepitopes: IEDB_130651,IEDB_136044,IEDB_136906,IEDB_137339,IEDB_137472,IEDB_1391964,IEDB_141794,IEDB_142487,IEDB_142488,IEDB_144987,IEDB_146664,IEDB_151528,IEDB_152217,IEDB_190606,IEDB_423106,IEDB_742247,IEDB_983931,SB_165,SB_166,SB_167,SB_178,SB_187,SB_192,SB_195,SB_27,SB_3,SB_31,SB_5,SB_6,SB_62,SB_7,SB_88
The structure is contained in the following publication(s):
- Article ID: 11836
Drøbak BK, Brewin NJ, Hernandez LE "Extraction, separation, and analysis of plant phosphoinositides and complex glycolipids" -
Methods in Molecular Biology 141 (2000) 157-174
glycolipids, HPLC, TLC, HPTLC, phosphoinositides, biotinylation
NCBI PubMed ID: 10820743Publication DOI: 10.1385/1-59259-067-5:157Journal NLM ID: 9214969Publisher: Springer
Institutions: Cell Signalling Group, Department of Cell Biology, John Innes Centre, Norwich, UK, Department of Genetics, John Innes Centre, Norwich, UK, Laboratory of Plant Physiology, Edificio de Biológicas, Universidad Autónoma de Madrid, Madrid, Spain
Expand this compound
Collapse this compound
Total list of structure IDs on all result pages of the current query:
Total list of corresponding CSDB IDs (record IDs):
Execution: 1 sec