Bradyrhizobium japonicum produces lipo-oligosaccharide signal molecules that induce deformation of root hairs and meristematic activity on soybeans. B. japonicum USDA135 (a Type I strain) produces modified chitin pentasaccharide molecules with either a terminal N-C16:0- or N-C18:1-glucosamine with and without an O-acetyl group at C-6 and with 2-O-methylfucose linked to C-6 of the reducing N-acetylglucosamine. An additional molecule has N-C16:1-glucosamine and no O-acetyl group. All of these molecules cause root hair deformation on Vicia sativa and Glycine soja. The C18:1-containing molecules were tested and found to induce meristem formation on G. soja. USDA61 (a Type II strain) produces eight additional molecules. Five have a carbamoyl group on the terminal N-acylglucosamine. Six have chitin tetrasaccharide backbones. Three have a terminal N-acyl-N-methylglucosaminosyl residue. In four molecules, the reducing-end N-acetylglucosamine is glycosidically linked to glycerol and has a branching fucosyl, rather than a 2-O-methylfucosyl, residue. One molecule has a terminal N-acylglucosamine that has both acetyl and carbamoyl groups (one each).
NCBI PubMed ID: 8349712Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Institutions: Complex Carbohydrate Research Center, University of Georgia, Athens 30602-4712, Department of Microbiology and the Center for Legume Research, University of Tennessee, Knoxville, lknnessee 37996, Institute of Molecular Plant Sciences, Leiden University, 2311 VJ Leiden, The Netherlands, Ohio State Biotechnology Center, Ohio State University, Columbus, Ohio 43210
Methods: 1H NMR, NMR-2D, FAB-MS, GC-MS, TLC, biological assays, composition analysis, NMR-1D
Found