Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Publication DOI: 10.1111/j.1365-313X.2005.02371.xJournal NLM ID: 9207397Publisher: Oxford: Blackwell Scientific Publishers and BIOS Scientific Publishers for the Society for Experimental Biology
Correspondence: ksaito
faculty.chiba-u.jp
Institutions: Department of Molecular Biology and Biotechnology, Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 263-8522, Japan, Institute of Life Science, Ehime Women's College, 421 Ibuki-cho Baba, Uwajima-shi, Ehime, 798-0025, Japan, CREST, JST (Japan Science and Technology Agency), Yayoi-cho 1-33, Inage-ku, Chiba-shi, Chiba 263-8522, Japan, RIKEN Plant Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, 230-0045, Japan, Phenomenome Discoveries Inc., 204-407 Downey Road, Saskatoon, SK S7N 4L8, Canada
The integration of metabolomics and transcriptomics can provide precise information on gene-to-metabolite networks for identifying the function of unknown genes unless there has been a post-transcriptional modification. Here, we report a comprehensive analysis of the metabolome and transcriptome of Arabidopsis thaliana over-expressing the PAP1 gene encoding an MYB transcription factor, for the identification of novel gene functions involved in flavonoid biosynthesis. For metabolome analysis, we performed flavonoid-targeted analysis by high-performance liquid chromatography-mass spectrometry and non-targeted analysis by Fourier-transform ion-cyclotron mass spectrometry with an ultrahigh-resolution capacity. This combined analysis revealed the specific accumulation of cyanidin and quercetin derivatives, and identified eight novel anthocyanins from an array of putative 1800 metabolites in PAP1 over-expressing plants. The transcriptome analysis of 22 810 genes on a DNA microarray revealed the induction of 38 genes by ectopic PAP1 over-expression. In addition to well-known genes involved in anthocyanin production, several genes with unidentified functions or annotated with putative functions, encoding putative glycosyltransferase, acyltransferase, glutathione S-transferase, sugar transporters and transcription factors, were induced by PAP1. Two putative glycosyltransferase genes (At5g17050 and At4g14090) induced by PAP1 expression were confirmed to encode flavonoid 3-O-glucosyltransferase and anthocyanin 5-O-glucosyltransferase, respectively, from the enzymatic activity of their recombinant proteins in vitro and results of the analysis of anthocyanins in the respective T-DNA-inserted mutants. The functional genomics approach through the integration of metabolomics and transcriptomics presented here provides an innovative means of identifying novel gene functions involved in plant metabolism.
glycosyltransferase, Arabidopsis thaliana, transcriptome, anthocyanins, metabolome, MYB factor
Structure type: oligomer ; 975 [M]+
Location inside paper: Figure 1
Trivial name: A5
Contained glycoepitopes: IEDB_114701,IEDB_116879,IEDB_142488,IEDB_146664,IEDB_167188,IEDB_174332,IEDB_983931,SB_192
Methods: FT-MS, enzymatic assay, genetic manipulations, HPLC/PDA/MS
Related record ID(s): 60860, 60861, 60862, 60863, 60864, 60865, 60867, 60868, 60869, 60870, 60871, 60872, 60873, 60874, 60875, 60876
NCBI Taxonomy refs (TaxIDs): 3702
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There is only one chemically distinct structure:
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