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1. (Article ID: 6090)
Kokoulin MS, Kuzmich AS, Romanenko LA, Chikalovets IV
Structure and in vitro antiproliferative activity of the acidic capsular polysaccharide from the deep-sea bacterium Psychrobacter submarinus KMM 225T
Carbohydrate Polymers 262 (2021)
117941
Psychrobacter submarinus KMM 225T is a Gram-negative bacterium isolated from a sea-water sample collected at a depth of 300 m in the Northwest Pacific Ocean. Here we report the structure of the capsular polysaccharide from P. submarinus KMM 225T and its effect on the viability and colony formation of cancer cells. The glycopolymer was purified by ultracentrifugation and chromatography methods, and the structure was elucidated using NMR spectroscopy and composition analyses. The following structure of the acidic capsular polysaccharide, containing 2-acetamido-2,4,6-trideoxy-4-[(S)-3-hydroxybutyramido]-d-glucose [d-QuipNAc4N(S-Hb)] and 4,6-O-[(S)-1-carboxyethylidene]-2-acetamido-2-deoxy-d-glucose [d-GlcpNAc4,6(S-Pyr)] was established: The capsular polysaccharide slightly reduced the viability but effectively suppressed the colony formation of different types of cancer cells, of which the most pronounced inhibition was shown for the human chronic myelogenous leukemia K-562 cells.
capsular polysaccharide, Marine bacterium, Antiproliferative activity, deep-sea, K-562, Psychrobacter submarinus
NCBI PubMed ID: 33838818Publication DOI: 10.1016/j.carbpol.2021.117941Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: maxchem
mail.ru
Institutions: G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 let Vladivostoku, Vladivostok, 690022, Russia, Far Eastern Federal University, 8, Sukhanova str., Vladivostok, 690950, Russia
Methods: 13C NMR, 1H NMR, NMR-2D, GC-MS, SDS-PAGE, sugar analysis, methanolysis, HPLC, HPSEC, cell viability assay, soft agar assay
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