Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Publication DOI: 10.1074/jbc.M611498200Journal NLM ID: 2985121RPublisher: Baltimore, MD: American Society for Biochemistry and Molecular Biology
Correspondence: ksaito
faculty.chiba-u.jp
Institutions: RIKEN Plant Science Center, Suehiro-cho 1-7-22, Tsurumi-ku, Yokohama 230-0045, Japan, Graduate School of Pharmaceutical Science, Chiba University, Yayoi-cho 1-33, Inage-ku, Chiba 263-8522, Japan
Glycosylation plays a major role in the remarkable chemical diversity of flavonoids in plants including Arabidopsis thaliana. The wide diversity encoded by the large family-1 glycosyltransferase (UGT) gene family makes it difficult to determine the biochemical function of each gene solely from its primary sequence. Here we used transcriptome coexpression analysis combined with a reverse genetics approach to identify a gene that is prominent in determining the flavonoid composition of Arabidopsis. Using transcriptome coexpression analysis accessible on the ATTED-II public data base, the expression pattern of a UGT gene, UGT89C1, was found to be highly correlated with known flavonoid biosynthetic genes. No C-7 rhamnosylated flavonols were detected in either of two T-DNA ugt89c1 mutants. This specific metabolite deficiency in the mutants was complemented by stable transformation with the genomic fragment containing intact UGT89C1. Glutathione S-transferasefused recombinant UGT89C1 protein converted kaempferol 3-O-glucoside to kaempferol 3-O-glucoside-7-O-rhamnoside and recognized 3-O-glycosylated flavonols and UDP-rhamnose as substrates, but not flavonol aglycones, 3-O-glycosylated anthocyanins or other UDP-sugars. These results show that UGT89C1 is a flavonol 7-O-rhamnosyltransferase. The abundance of UGT89C1 transcripts in floral buds was consistent with the flavonoid accumulation of C-7 rhamnosylated flavonols in Arabidopsis organs. Our present study demonstrates that the integration of transcriptome coexpression analysis with a reverse genetic approach is a versatile tool for understanding a multigene family of a metabolic pathway in Arabidopsis.
Structure type: oligomer
Location inside paper: Figure 4
Trivial name: f5
Compound class: glycoside, flavonoid glycoside, flavonol glycoside
Contained glycoepitopes: IEDB_136105,IEDB_225177,IEDB_885823
Methods: biological assays, enzymatic assay, genetic manipulations, UPLC/PDA/ESI-Q-TOF/MS
Related record ID(s): 60952, 60953, 60954, 60955, 60957, 60958, 60959
NCBI Taxonomy refs (TaxIDs): 3702Reference(s) to other database(s): CCSD:
49907, CBank-STR:2107
Show glycosyltransferases
There is only one chemically distinct structure:
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