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1. (Article ID: 6155)
Vieira AM, Zahed F, Crispim AC, de Souza Bento E, França RFO, Pinheiro IO, Pardo LA, Carvalho BM
Production of levan from Bacillus subtilis var. natto and apoptotic effect on SH-SY5Y neuroblastoma cells
Carbohydrate Polymers 273 (2021)
118613
Levan is a high-valued polysaccharide of fructose produced by several microbial species. These polysaccharides have been described as effective therapeutic agents in some human disease conditions, such as cancer, heart diseases and diabetes. The objective of this study was to examine the effect of levan (β-(2→6)-fructan) produced through sucrose fermentation by B. subtilis var. natto on the proliferation rate, cytotoxicity, and apoptosis of human neuroblastoma SH-SY5Y cells. It was obtained 41.44 g/L of levan in 18 h by biotechnological fermentation and SH-SY5Y cells were exposed to 1000 μg/mL of levan. The treatment with 1000 μg/mL of levan induced apoptosis in SH-SY5Y cancer cells by the significant increase in Annexin V/7-AAD and caspase 3/7 activation, but did not decrease proliferation or triggered a cytotoxic effect. 1000 μg/mL levan treatment is a promising therapeutic strategy for SH-SY5Y neuroblastoma cells
exopolysaccharide, live-cell analysis, neoplastic cells
NCBI PubMed ID: 34561011Publication DOI: 10.1016/j.carbpol.2021.118613Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: B.M. Carvalho
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Institutions: Biological Sciences Institute, University of Pernambuco (ICB/UPE), Recife, Brazil, Max Planck Institute for Experimental Medicine, Göttingen, Germany, Institute of Chemistry and Biotechnology, Federal University of Alagoas, Maceio, Brazil, Department of Virology, Aggeu Magalh˜aes Institute (IAM/FIOCRUZ Pernambuco), Recife, Brazil
Methods: 13C NMR, 1H NMR, NMR-2D, statistical analysis, fermentation, cell proliferation assay, cytotoxic analysis, apoptosis assays
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