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Acinetobacter baumannii isolate LUH5552 carries the KL89 capsule biosynthesis gene cluster. Capsular polysaccharide (CPS) isolated from LUH5552 was analyzed by sugar analysis, Smith degradation, and one- and two-dimensional 1H and 13C NMR spectroscopy. The K89 CPS structure has not been seen before in A. baumannii CPS structures resolved to date and includes a 3-acetamido-3,6-dideoxy-D-galactose (D-Fucp3NAc) residue which is rare amongst A. baumannii CPS. The K89 CPS has a →3)-α-D-GalpNAc-(1→3)-β-D-GlcpNAc-(1→ main chain with a β-D-Glcp-(1→2)-β-D-Fucp3NAc-(1→6)-D-Glcp side branch that is α-(1→4) linked to D-GalpNAc. The roles of the Wzy polymerase and the four glycosyltransferases encoded by the KL89 gene cluster in the biosynthesis of the K89 CPS were assigned. Two glycosyltransferases, Gtr121 and Gtr122, link the d-Fucp3NAc to its neighboring sugars.

Acinetobacter baumannii, 6-dideoxy-D-galactose, polysaccharide structure, 3-Acetamido-3, capsule, K locus

NCBI PubMed ID: 35843396
Publication DOI: 10.1016/j.ijbiomac.2022.07.085
Journal NLM ID: 7909578
Publisher: Butterworth-Heinemann
Correspondence: johanna.kenyon

qut.edu.au
Institutions: N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia, M. M. Shemyakin & Y. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia, State Research Center for Applied Microbiology and Biotechnology, Obolensk, Moscow Region, Russia, Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region, Russia, School of Life and Environmental Sciences, The University of Sydney, Sydney, Australia, Centre for Immunology and Infection Control, School of Biomedical Sciences, Faculty of Health, Queensland University of Technology, Brisbane, Australia
Methods: 13C NMR, 1H NMR, NMR-2D, sugar analysis, GLC, Smith degradation, GPC, bioinformatic analysis, NaBH4 reduction