Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Publication DOI: 10.1080/07328309708007337Journal NLM ID: 8218151Publisher: Marcel Dekker
Institutions: Dipartimento BBCM, University of Trieste, Trieste, Italy
The solution properties of the exopolysaccharide marginalan produced by Pseudomonas marginalis HT041B were investigated by means of low-angle laser light-scattering, capillary viscometry, and rheology. Potentiometric and viscosimetric data indicated the absence of a cooperative transition of the disorder-to-order type. The experimental findings obtained in dilute solution (Mark-Houwink coefficients, rigidity coefficient, characteristic ratio) suggested that the polymer behaves like a semiflexible chain which adopts a disordered conformation. The rheological behaviour of more concentrated marginalan solutions, as determined by means of both steady shear and oscillatory measurements, further confirmed the disordered conformational state of the polymer in solution.
strain, Pseudomonas, property, exopolysaccharide, solution, solution properties, Pseudomonas marginalis
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 1
Trivial name: marginalan
Contained glycoepitopes: IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_151528,IEDB_151770,IEDB_151771,IEDB_190606,IEDB_983931,SB_192,SB_7
Methods: light scattering, rheological measurements, capillary viscosimetry
3D data: solution properties
NCBI Taxonomy refs (TaxIDs): 298
Show glycosyltransferases
There is only one chemically distinct structure:
Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
NCBI PubMed ID: 32255886Publication DOI: 10.1016/j.foodhyd.2019.105446Journal NLM ID: 8701770Publisher: New York, NY: Elsevier
Correspondence: Wolf B <B.Wolf
bham.ac.uk>; Harding SE <steve.harding
nottingham.ac.uk>
Institutions: National Centre for Macromolecular Hydrodynamics, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, UK, Division of Food, Nutrition and Dietectics, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, UK, School of Health Sciences, University of Nottingham, Queen's Medical Centre, Nottingham, UK, Nofima AS, Norwegian Institute of Food, Fisheries and Aquaculture Research, Ås, Norway, Kulturhistorisk Museum, Universitetet i Oslo, Oslo, Norway
Scleroglucan, a neutral β(1-3) glucan with β(1-6) glucan branches every third residue, is being considered as an alternative rod-like, shear thinning high molecular weight β-glucan based polysaccharide to xanthan gum for the management of patients with oropharyngeal dysphagia. It is therefore important to understand more fully its hydrodynamic properties in solution, in particular heterogeneity, molecular weight distribution and its behaviour in the presence of mucin glycoproteins. A commercially purified scleroglucan preparation produced by fermentation of the filamentous fungus Sclerotium rolfsii was analysed in deionised distilled water with 0.02% added azide. Sedimentation velocity in the analytical ultracentrifuge showed the scleroglucan preparation to be unimodal at concentrations >0.75 mg/ml which resolved into two components at lower concentration and with partial reversibility between the components. Sedimentation coefficient versus concentration plots showed significant hydrodynamic non-ideality. Self-association behaviour was confirmed by sedimentation equilibrium experiments with molecular weights between ~3000000 g/mol to ~5000000 g/mol after correcting for thermodynamic non-ideality. SEC-MALS-viscosity experiments showed a transition between a rod-shape at lower molar masses to a more flexible structure at higher masses consistent with previous observations. Sedimentation velocity experiments also showed no evidence for potentially problematic interactions with submaxillary mucin.
viscosity, scleroglucan, Sclerotium rolfsii, molecular properties
Structure type: polymer chemical repeating unit
Location inside paper: Fig. 1
Trivial name: β-D-glucan, β-glucan, schizophyllan, carboxymethylglucan, pleuran, scleroglucan, lentinan, GRN, SPG, sizofiran, shizophyllan, Scleroglucan, lentinan-type beta-glucans (Ths-2), b-(1-3,6)-glucan, HEP3, grifolan LE, schizophyllan, scleroglucan, lentinan, termitan, grifolan, schizophyllan, scleroglucan, schizophyllan, sizofiran, grifolan, scleroglucan, schizophyllan, sonifilan, schizophyllan, grifolan, G. frondosa polysaccharide (GFP), alkali-soluble β-glucan (PeA3), schizophyllan (SPG), β-glucan, schizophyllan, β-glucan
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide, β-glucan, b-glucan, D-glucan, scleroglucan
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: capillary viscosimetry, dialysis, SEC-MALLS, centrifugation
Related record ID(s): 42445, 42454, 43179, 48394, 48426, 48733, 50040, 50627, 50649, 50652
NCBI Taxonomy refs (TaxIDs): 39291Reference(s) to other database(s): GTC:G66305IS, CCSD:
49943, CBank-STR:12679, CA:9050-67-3
Show glycosyltransferases
There is only one chemically distinct structure:
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