CSDB: Search results

Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Pseudomonas aeruginosa [ICD11: XN5L6

]

NCBI PubMed ID: 9848676
Journal NLM ID: 8204476
Publisher: Wiley-VCH
Correspondence: pierre.thibault

nrc.ca
Institutions: Institute for Biological Sciences, Ottawa, Ontario, Canada

The analysis of underivatized core oligosaccharides arising from mild acid hydrolysis of lipopolysaccharides from Pseudomonas aeruginosa serotype 05 was achieved using a transient isotachophoretic preconcentration method coupled to capillary zone electrophoresis-electrospray-mass spectrometry (tCITP-CZE-ES-MS). The combination of a tCITP preconcentration step provided a 10- to 50-fold enhancement of sample loading and a corresponding improvement in sensitivity compared to the conventional zone electrophoresis format. Electrophoretic conditions, enabling the separation of these anionic analytes, were developed to determine possible sites of heterogeneity on either the core or the O-chain structures. The tCITP-CZE-ES-MS technique provided unparalleled resolution of the different core glycoforms and oligosaccharides obtained from the acid cleavage of the native endotoxins whether isolated following conventional gel permeation chromatography or obtained from direct hydrolysis of the bacterial isolates. These investigations also highlighted the highly phosphorylated nature of these complex cell membrane components, where the heptose residues of the core oligosaccharide can bear up to six phosphate groups.

Pseudomonas aeruginosa, Oligosaccharides, Capillary zone electrophoresis, electrospray mass spectrometry

Structure type: oligomer
Location inside paper: Fig. 1
Aglycon: lipid A
Compound class: core oligosaccharide
Contained glycoepitopes: IEDB_130650,IEDB_130659,IEDB_136105,IEDB_137473,IEDB_140088,IEDB_142488,IEDB_144144,IEDB_144998,IEDB_146664,IEDB_2189047,IEDB_225177,IEDB_885823,IEDB_983931,SB_192

Methods: CE-ESI-MS, transient isotachophoresis
Comments, role: lipopolysaccharide core, distribution of phosphate groups has been corrected later

Related record ID(s): 6390
NCBI Taxonomy refs (TaxIDs): 287
Show glycosyltransferases

There are 2 chemically distinct structures. Please, select:

C[C@H](N)C(=O)N[C@H]1[C@@H](O[C@@H]2[C@H](O)[C@@H](O[C@@H]3[C@H](OP(=O)(O)O)[C@@H](O[C@@H]4[C@H](O[C@]5(C(=O)O)C[C@@H](O)[C@@H](O)[C@@H]([C@H](O)CO)O5)C[C@](O)(C(=O)O)O[C@@H]4[C@H](O)CO)O[C@H]([C@H](CO)OP(=O)(O)O)[C@H]3OP(=O)(O)O)O[C@H]([C@@H](O)CNC(=O)O)[C@H]2O)O[C@H](CO)[C@H](O[C@H]2O[C@H](CO[C@@H]3O[C@@H](C)[C@H](O)[C@@H](O)[C@H]3O[C@@H]3O[C@H](CO)[C@@H](O)[C@H](O)[C@H]3O)[C@@H](O)[C@H](O)[C@H]2O)[C@@H]1O[C@@H]1O[C@H](CO[C@H]2O[C@H](CO)[C@@H](O)[C@H](O)[C@H]2O)[C@@H](O)[C@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize

Taxonomic group: bacteria / Proteobacteria (Phylum: Proteobacteria)
Associated disease: infection due to Pseudomonas aeruginosa [ICD11: XN5L6

]

NCBI PubMed ID: 9848676
Journal NLM ID: 8204476
Publisher: Wiley-VCH
Correspondence: pierre.thibault

nrc.ca
Institutions: Institute for Biological Sciences, Ottawa, Ontario, Canada

Pseudomonas aeruginosa, Oligosaccharides, Capillary zone electrophoresis, electrospray mass spectrometry

Structure type: polymer chemical repeating unit
Location inside paper: Fig. 1
The structure in this paper was incorrect:

Original structure from CSDB record 6390

← originally published structure

Show legend

Compound class: O-polysaccharide

Methods: CE-ESI-MS, transient isotachophoresis

Related record ID(s): 6229
NCBI Taxonomy refs (TaxIDs): 287
Reference(s) to other database(s): GlycomeDB:33658
Show glycosyltransferases

There is only one chemically distinct structure:

[*]O[C@@H]1[C@@H](C(=O)O)O[C@@H](O[C@@H]2[C@@H](C(=O)O)O[C@@H](O[C@@H]3[C@@H](NC(C)=O)[C@@H]([*])O[C@H](C)[C@@H]3O)[C@@H](NC(C)=O)[C@H]2NC(C)=O)[C@@H](NC(C)=N)[C@H]1NC(C)=O

SVG MW SMILES 3D mol Ionize