Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: root
NCBI PubMed ID: 10226587Journal NLM ID: 8102988WWW link: https://www.webofscience.com/wos/woscc/full-record/WOS:000079786100071Publisher: Attiki, Greece: International Institute of Anticancer Research
Institutions: College of Pharmacy, Seoul National University, Seoul 151742, Korea
In this paper, we present evidence that Ginsenoside-Rs(3) (G-Rs3), a new diol-type ginseng saponin isolated from the roots of Panax ginseng C.A. Meyer, efficiently arrests the cell cycle at the G1/S boundary at lower doses, 0.1-5 μM, but induces apoptosis at higher doses, 10-25 μM, the effects of which were associated with selectively elevating protein levels of p53 and p21(WAF1) in SK-HEP-1 cells. The cell growth suppressive and apoptosis inducing effects were confirmed by MTT assays together with flow cytometric analyses, morphological changes and DNA fragmentation. Immunoblotting showed that G-Rrs3 significantly elevated protein levels of p53 and p21(WAF1) prior to inducing apoptosis, while it did not elevate those of cyclin E, cyclin A, p27(Kip1), and PCNA. Immune complex kinase assays showed that G-Rs3 downregulated the activities of both cyclins E- and A-associated kinases. Collectively, we suggest that G-Rs3 selectively elevates protein levels of p53 and p21 and hence downregulates the activities of the cyclin-dependent kinases, resulting in cell cycle nn est at the G1/S boundary. We also propose that apoptosis induced by G-Rs3 is related to the elevations of p53 and p21(WAF1) in the cells.
apoptosis, p53, ginsenoside-Rs(3), p21(WAF1), SK-HEP-1 cells
Structure type: oligomer
Location inside paper: ginsenoside Rs3, G-Rs3
Trivial name: ginsenoside Rs3
Compound class: saponin glycoside, glycoside
Contained glycoepitopes: IEDB_140628,IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: DNA techniques, immunoblotting, morphological analysis, flow cytometry analysis, MTT assay, immune complex kinase assay
Biological activity: G-Rs3 arrests cell cycle at the G1/S boundary at 0.1-5 μM, induces apoptosis at 10-25 μM and elevated protein levels of p53 and p21(WAF1) in SK-HEP-1 cells.
Comments, role: full text of article is not available, record was created with help of WOS database, structure was reproduced according to Pubchem/ChemSpider
NCBI Taxonomy refs (TaxIDs): 4054
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
report error