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1. (Article ID: 6253)
 
Kasimova AA, Schneider MM, Edelstein MV, Dzhaparova AA, Shashkov AS, Knirel YA, Kenyon JJ
Structure of the K98 capsular polysaccharide from Acinetobacter baumannii REV-1184 containing a cyclic pyruvic acid acetal
International Journal of Biological Macromolecules 218 (2022) 447-455
 

The K98 capsular polysaccharide (CPS) from the Acinetobacter baumannii clinical isolate, REV-1184, was studied by sugar analysis and Smith degradation along with one- and two-dimensional 1H and 13C NMR spectroscopy and high-resolution electrospray ionization mass spectrometry. The CPS was found to consist of linear tetrasaccharide repeats (K-units) that include one residue each of D-GlcpNAc, D-GalpNAc, 2-acetamido-2-deoxy-D-galacturonic acid (D-GalpNAcA), and 2-acetamido-2,6-dideoxy-D-glucose (N-acetylquinovosamine, D-QuipNAc), with the GalpNAc residue decorated with a (R)-configurated 4,6-pyruvic acid acetal group. The CPS has a similar composition to that of A. baumannii K4 but the topology of the tetrasaccharide K-unit is different (linear in K98 versus branched in K4). This was due to a difference in sequence for the Wzy polymerases encoded by the CPS biosynthesis gene clusters KL98 and KL4, with the WzyK98 polymerase forming a β-D-QuipNAc-(1→3)-D-GalpNAc linkage between the K98 units.

Acinetobacter baumannii, capsular polysaccharide, 2-acetamido-2, 6-dideoxy-d-glucose, 2-acetamido-2-deoxy-D-galacturonic acid, Pyruvic acid acetal, K locus

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