The structure of the O-specific polysaccharide (OPS) from Aeromonas encheleia strain A4 lipopolysaccharide was investigated. A. encheleia strain A4, classified into the new provisional serogroup PGO1 predominating among aeromonads in Polish aquaculture, was isolated from common carp tissues during an outbreak of MAI/MAS disease on a fish farm. The high-molecular-weight OPS fraction liberated from the lipopolysaccharide after mild acid hydrolysis followed by gel-permeation chromatography was studied with chemical methods, mass spectrometry, and one- and two-dimensional 1H and 13C NMR spectroscopy techniques. Inter-residue correlations were identified in 1H,13C-heteronuclear multiple-bond correlation (HMBC) and 1H,1H NOESY experiments. It was found that the O-specific polysaccharide of A. encheleia strain A4 consists of branched pentasaccharide repeating units with the following structure:→2)[α-d-Fucp3NRHb-(1→3)]-α-l-Rhap-(1→3)-β-l-Rhap-(1→4)-α-l-Rhap-(1→3)-β-d-QuipNAc-(1→.

Lipopolysaccharide, O-antigen, NMR spectroscopy, O-specific polysaccharide, Aeromonas encheleia, Fucp3N

• Compound ID: 16172
  

  R-3HOBut-(1-3)-a-D-Fucp3N-(1-3)-+ | -2)-a-L-Rhap-(1-3)-b-L-Rhap-(1-4)-a-L-Rhap-(1-3)-b-D-QuipNAc-(1-

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  Structure type: polymer chemical repeating unit
  Compound class: O-polysaccharide, O-antigen
  
   
  
  Aeromonas encheleia PGO1 A4
  CSDB ID 8493 (all data & tools)