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1. (Article ID: 6282)
 
Nasir A, Ahmad W, Sattar F, Ashfaq I, Lindemann SR, Chen MH, Van den Ende W, Oner ET, Kirtel O, Khaliq S, Ghauri MA, Anwar MA
Production of a high molecular weight levan by Bacillus paralicheniformis, an industrially and agriculturally important isolate from the buffalo grass rhizosphere
Antonie van Leeuwenhoek 115(9) (2022) 1101-1112
 

A new exopolysaccharide (EPS) producing Gram-positive bacterium was isolated from the rhizosphere of Bouteloua dactyloides (buffalo grass) and its EPS product was structurally characterized. The isolate, designated as LB1-1A, was identified as Bacillus paralicheniformis based on 16S rRNA gene sequence and phylogenetic tree analysis. The EPS produced by LB1-1A was identified as a levan, having β(2→6) linked backbone with β(2→1) linkages at the branch points (4.66%). The isolate LB1-1A yielded large amount (~ 42 g/l) of levan having high weight average molecular weight (Mw) of 5.517 × 107 Da. The relatively low degree of branching and high molecular weight of this levan makes B. paralicheniformis LB1-1A a promising candidate for industrial applications.

exopolysaccharide, fructan, levan, Bacillus paralicheniformis, fructooligosaccharide

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