The structure of an oligosaccharide and its effect on cultured plant cells are reported in this paper. The oligosacchride with different DP (degree of polymerization) were isolated from acid-hydrolyzed cell walls of cultured cells from Panax ginseng. The samples were passed through an active carbon column, a Dowex (H+) column, and a Bio-Gel P-2 column and finally separated by HPLC. The oligosaccharide with different DP were obtained. The possible structure of an oligosaccharide DP-6 was characterized by TGC, GC-MS, FAB-MS and 13C-NMP, measurements. Experiments showed that this oligosaccharide could increase the growth rate of Taxus yunnanensis cultured cells by 36.7% and promote the taxol yield by nearly 2 folds. Its effect was also found in other cultured plant cells such as panax notoginseng and Carthamus tinctorius.

oligosaccharide, effect, cultured plant cells

1H NMR data:
missing...

There is only one chemically distinct structure:

SVGMWSMILES3D molIonize

 

OC[C@H]1O[C@@H](O[C@@H]2[C@@H](CO)O[C@@H](O[C@@H]3[C@@H](CO)O[C@@H](O[C@H]4[C@H](O)[C@@H](O[C@H]5O[C@H](CO)[C@H](O)[C@H](O)[C@H]5O)[C@H](O[C@@H]5[C@@H](CO)O[C@@H](O)[C@@H](O)[C@H]5O)O[C@@H]4CO)[C@H](O)[C@H]3O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@@H]1O

990.861 g/mol (C₃₆H₆₂O₃₁)