Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: twig,
leaf
NCBI PubMed ID: 10630118Publication DOI: 10.1055/s-2006-960854Journal NLM ID: 0066751Publisher: George Thieme
Correspondence: Tan RX <rxtan
netra.nju.edu.cn>
Institutions: Department of Biochemistry, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong, China, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing, China, Institute of Pharmacognosy & Phytochemistry, School of Pharmacy, University of Lausanne, BEP, Lausanne-Dorigny, Switzerland
Xanthine oxidase is a key enzyme associated with the incidence of hyperuricemia-related disorders. Repeated chromatography of the enzyme inhibitory part of the water extract of the twigs and leaves of Brandisia hancei (Scrophulariaceae) gave a flavone luteolin, an iridoid glycoside mussaenoside, two β-sitosterol glycosides daucosterol and β-sitosterol gentiobioside, and five phenylethanoids arenarioside, brandioside, acteoside, 2'-O-acetylacteoside and isoacteoside. Luteolin and isoacteoside inhibited the xanthine oxidase (XO, EC 1.2.3.2) with the IC50 values at 7.83 and 45.48 μM, respectively. Isoacteoside was found to be the first phenylethanoid that decreased substantially the formation of uric acid by inhibiting competitively xanthine oxidase (K(i) value: 10.08 μM). Furthermore, the study suggested that the caffeoylation of the 6'-hydroxyl group of the phenylethanoids was essential for the enzyme inhibitory action.
inhibitor, Brandisia hancei, xanthine oxidase
Structure type: monomer
Location inside paper: daucosterol
Trivial name: daucosterol, β-daucosterol, β-sitosterol, β-sitosterol-β-D-glucoside, androsine, saxifragifolin B, β-sitosterol 3-O-β-D-glucopyranoside
Compound class: saponin glycoside, glycoside, steroid glycoside, triterpene glycoside, triterpenoid glycoside
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: 13C NMR, 1H NMR, FAB-MS, inhibition studies, TLC, MS, HPLC, UV, optical rotation measurement, HMBC, HMQC, DEPT, COSY, LC-UV
Related record ID(s): 63022, 63023, 63024, 63025, 63026, 63028, 63029
NCBI Taxonomy refs (TaxIDs): 255883Reference(s) to other database(s): GenDB:MK026953.1
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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