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1. (Article ID: 6332)
Wagh VV, Said MS, Bennale JS, Dastager SG
Isolation and structural characterization of exopolysaccharide from marine Bacillus sp. and its optimization by Microbioreactor
Carbohydrate Polymers 285 (2022)
119241
In the present study, a cost-effective, robust Microbioreactor based production optimization of levan like exopolysaccharide from marine Bacillus sp. SGD-03 was analysed. FE-SEM analysis has showed the significant fibrillar structure of EPS. Size exclusion chromatography and other analytical data revealed that, produced EPS has a molecular weight of 1.0 × 104 Da and is composed of fructose monosaccharide with hydroxyl, carbonyl, and ether groups. The backbone structure of EPS has a branching pattern of β-(2,6) linkages which confirms the similarity with available levan like polymers. The cost-effective media composition for levan production was demonstrated. The maximum yield of crude levan obtained was 123.9 g/L by response surface methodology using robust BioLector Pro Microbioreactor, and same has been validated with shake flask, 1 L and 10 L pilot-scale fermentation.
structure, EPS, marine, optimization, microbioreactor
NCBI PubMed ID: 35287863Publication DOI: 10.1016/j.carbpol.2022.119241Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: S.G. Dastager
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Institutions: Academy of Scientific and Innovative Research (AcSIR), New Delhi, India, NCIM Resource Center, CSIR-National Chemical Laboratory, Pune 411008, India, Division of Organic Chemistry, CSIR-National Chemical Laboratory, Pune 411008, India, Beckman Coulter Life Sciences, Mumbai, India
Methods: 13C NMR, 1H NMR, NMR-2D, PCR, sugar analysis, TLC, FTIR, SEC-HPLC, SEM, DEPT, genome sequencing, UHPLC, FE-SEM, EPS production, data analysis, screening of EPS producing strain, LC-HRMS
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