Aeromonas sobria strain K928 was isolated from a common carp during a Motile Aeromonas Infection/Motile Aeromonas Septicaemia disease outbreak on a Polish fish farm and classified into the new provisional PGO1 serogroup. The lipopolysaccharide of A. sobria K928 was subjected to mild acid hydrolysis, and the O-specific polysaccharide, which was isolated by gel-permeation chromatography, was studied using sugar and methylation analyses and 1H and 13C NMR spectroscopy. The following structure of the branched O-specific polysaccharide repeating unit of A. sobria K928 was established. →2)[α-D-Fucp3NRHb-(1→3)]-α-L-Rhap-(1→3)-β-L-Rhap-(1→4)-α-L-Rhap-(1→3)-β-D-FucpNAc-(1→ The O-antigen gene cluster was identified and characterized in the genome of the A. sobria K928 strain after comparison with sequences in the available databases. The composition of the O-antigen genetic region was found to be consistent with the O-polysaccharide structure, and its organization was proposed.

O-antigen, NMR spectroscopy, O-antigen gene cluster, Aeromonas, Wzx/Wzy pathway

• Compound ID: 16539
  

  R-3HOBut-(1-3)-a-D-Fucp3N-(1-3)-+ | -2)-a-L-Rhap-(1-3)-b-L-Rhap-(1-4)-a-L-Rhap-(1-3)-b-D-FucpNAc-(1-

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  Structure type: polymer chemical repeating unit
  Compound class: O-polysaccharide, O-antigen
  
   
  
  Aeromonas sobria PGO1 K928
  CSDB ID 22268 (all data & tools)