hree kinds of novel cyclic isomaltooligosaccharides were isolated from the culture broth of a strain of Bacillus sp. T-3040, which had been isolated from soil. They were identified as cycloisomalto-heptaose, -octaose, and -nonaose, respectively, on the basis of their mass spectra, proton nuclear magnetic resonance spectra, carbon nuclear magnetic resonance spectra, infrared spectra, reducing power, and results of enzymatic analysis using endo-dextranase and exo-dextranase. They also showed features similar to cyclodextrins upon high-pressure liquid chromatography analysis using an ODS column.
NCBI PubMed ID: 27281012Publication DOI: 10.1271/bbb.57.1225Journal NLM ID: 9205717Publisher: Japan Society for Bioscience, Biotechnology, and Agrochemistry
Institutions: Noda Institute for Scientific Research, 399 Noda, Noda-shi, Chiba 278, Japan, National Food Research Institute, Ministry of Agriculture, Forestry, and Fisheries, 2-1-2 Kannondai, Tsukuba, Ibaraki 305, Japan
Methods: 13C NMR, 1H NMR, IR, MS, HPLC, UV