Taxonomic group: fungi / Basidiomycota
(Phylum: Basidiomycota)
Organ / tissue: sclerotium
NCBI PubMed ID: 11093721Publication DOI: 10.1016/s0008-6215(00)00140-3Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: Huibi X <hbxu

mail.hust.edu.cn>
Institutions: Department of Chemistry, Huazhong University of Science and Technology, China
An alkali-soluble polysaccharide (Hunai polysaccharide, 1) from the fruit body of Pleurotus tuber-regium (Fr.) Sing was shown to be homogeneous by gel permeation chromatography and its molecular weight was approximately 430000. Complete acid hydrolysis, periodate oxidation, Smith degradation, methylation, FT-IR and 13C NMR analysis, complex formation with Congo Red, indicated that 1 has a β-(1→3)-linked D-glucopyranosyl backbone with a single beta-D-glucopyranosyl group at O-6 of every third glucose residue.
polysaccharide, structural studies, Pleurotus tuber-regium, (1→3)-β-D-glucan
Structure type: structural motif or average structure ; 430000
Location inside paper: abstract
Compound class: glucan, polysaccharide
Contained glycoepitopes: IEDB_1397514,IEDB_141806,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_161166,IEDB_241101,IEDB_558869,IEDB_857743,IEDB_983931,SB_192
Methods: 13C NMR, methylation, GC-MS, acid hydrolysis, GLC, Smith degradation, GPC, UV, extraction, periodate oxidation, optical rotation measurement, acetylation, elemental analysis, reduction, dialysis, Sevag method, phenol-sulfuric acid method, Congo Red
NCBI Taxonomy refs (TaxIDs): 716892
Show glycosyltransferases
There is only one chemically distinct structure: