Detailed information was obtained regarding the partial structure of the lipopolysaccharide (LPS), containing glucose, glucuronic acid, 2-amino-2-deoxy-glucose, l-glycero-d-gluco-heptose, and small proportions of l-glycero-d-manno-heptose, mannose, and galactose, isolated from Vibrio cholera Ogawa G-2102. Structures of three oligosaccharides were determined. Results of deamination experiments established the sequence of the linkages between the amino sugar and heptose residues in the O-antigenic polysaccharide.
Publication DOI: 10.1016/0008-6215(83)88332-3Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Department of Macromolecules, Indian Association for the Cultivation of Science, Calcutta-700032 India, National Institute of Cholera and Enteric Diseases, 3 Kyd Street, Calcutta-700016 India
Methods: methylation, sugar analysis, acid hydrolysis, GLC, paper chromatography, de-N-acetylation, ion-exchange chromatography, optical rotation measurement, reduction