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Framm JJ, Peterson A, Thoeringer C, Pangert A, Hornung E, Feussner I, Luckner M, Lindemann P
Cloning and functional expression in Escherichia coli of a cDNA encoding cardenolide 16'-O-glucohydrolase from Digitalis lanata Ehrh.
Plant and Cell Physiology 41(11) (2000)
1293-1298
|
b-D-Glcp-(1-4)-b-D-Digp3Ac-(1-4)-b-D-Digp-(1-4)-b-D-Digp-(1-3)-Subst
Subst = digoxigenin = SMILES O=C1OCC([C@H]2CC{14}[C@]3(O)[C@]4([H])CC[C@]5([H])C{3}[C@@H](O)CC[C@]5(C)[C@@]4([H])C{12}[C@@H](O)[C@]23C)=C1 |
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Digitalis lanata
(NCBI TaxID 49450,
species name lookup)
Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: leaf
NCBI PubMed ID: 11092916Publication DOI: 10.1093/pcp/pcd060Journal NLM ID: 9430925Publisher: Tokyo: Oxford University Press
Correspondence: luckner
pharmazie.uni-halle.de
Institutions: Institut für Pharmazeutische Biologie, Martin-Luther-Universität, Halle (Saale), Germany, Biozentrum, Halle (Saale), Germany, Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany
A clone of cardenolide 16'-O-glucohydrolase cDNA (CGH I) was obtained from Digitalis lanata which encodes a protein of 642 amino acids (calculated molecular mass 73.2 kDa). The amino acid sequence derived from CGH I showed high homology to a widely distributed family of beta-glucohydrolases (glycosyl hydrolases family 1). The recombinant CGH I protein produced in Escherichia coli had CGH I activity. CGH I mRNA was detected in leaves, flowers, stems and fruits of D. lanata.
Scrophulariaceae, Digitalis lanata, glucosyl hydrolase (EC 3.2.1.21), lanatoside
Structure type: oligomer
Trivial name: lanatoside C
Compound class: saponin glycoside, glycoside, cardiac glycoside
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: PCR, DNA sequencing, DNA cloning, SDS-PAGE, amino acid analysis, enzymatic digestion, extraction, chromatography, isotopic labeling, cell growth, sonication, centrifugation, BLAST, FASTA
Related record ID(s): 65494
NCBI Taxonomy refs (TaxIDs): 49450Reference(s) to other database(s): CCSD:
45696, CBank-STR:8793
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Framm JJ, Peterson A, Thoeringer C, Pangert A, Hornung E, Feussner I, Luckner M, Lindemann P
Cloning and functional expression in Escherichia coli of a cDNA encoding cardenolide 16'-O-glucohydrolase from Digitalis lanata Ehrh.
Plant and Cell Physiology 41(11) (2000)
1293-1298
|
b-D-Digp3Ac-(1-4)-b-D-Digp-(1-4)-b-D-Digp-(1-3)-Subst
Subst = digoxigenin = SMILES O=C1OCC([C@H]2CC{14}[C@]3(O)[C@]4([H])CC[C@]5([H])C{3}[C@@H](O)CC[C@]5(C)[C@@]4([H])C{12}[C@@H](O)[C@]23C)=C1 |
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Digitalis lanata
(NCBI TaxID 49450,
species name lookup)
Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: leaf
NCBI PubMed ID: 11092916Publication DOI: 10.1093/pcp/pcd060Journal NLM ID: 9430925Publisher: Tokyo: Oxford University Press
Correspondence: luckner
pharmazie.uni-halle.de
Institutions: Institut für Pharmazeutische Biologie, Martin-Luther-Universität, Halle (Saale), Germany, Biozentrum, Halle (Saale), Germany, Institut für Pflanzengenetik und Kulturpflanzenforschung, Gatersleben, Germany
A clone of cardenolide 16'-O-glucohydrolase cDNA (CGH I) was obtained from Digitalis lanata which encodes a protein of 642 amino acids (calculated molecular mass 73.2 kDa). The amino acid sequence derived from CGH I showed high homology to a widely distributed family of beta-glucohydrolases (glycosyl hydrolases family 1). The recombinant CGH I protein produced in Escherichia coli had CGH I activity. CGH I mRNA was detected in leaves, flowers, stems and fruits of D. lanata.
Scrophulariaceae, Digitalis lanata, glucosyl hydrolase (EC 3.2.1.21), lanatoside
Structure type: oligomer
Trivial name: α-acetyldigoxin
Compound class: glycoside, cardiac glycoside
Methods: PCR, DNA sequencing, DNA cloning, SDS-PAGE, amino acid analysis, enzymatic digestion, extraction, chromatography, isotopic labeling, cell growth, sonication, centrifugation, BLAST, FASTA
Related record ID(s): 65493
NCBI Taxonomy refs (TaxIDs): 49450
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