Show legend Show as text

Structure type: polymer chemical repeating unit
Trivial name: succinoglycan
Compound class: EPS
Contained glycoepitopes: IEDB_135614,IEDB_136044,IEDB_137472,IEDB_141794,IEDB_141806,IEDB_142487,IEDB_142488,IEDB_146664,IEDB_153543,IEDB_158555,IEDB_190606,IEDB_241101,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_6,SB_7,SB_88

• Article ID: 2963
  Matulová M, Toffanin R, Navarini L, Gilli R, Paoletti S, Cesaro A "NMR analysis of succinoglycans from different microbial sources: partial assignment of their 1H and 13C NMR spectra and location of the succinate and the acetate groups" - Carbohydrate Research 265 (1994) 167-179
  

  In order to obtain information on the location of succinate and acetate groups, comparative NMR analyses were carried out on succinoglycans from different microbial sources by using conventional and advanced NMR techniques. In particular, one-dimensional, 1H and 13C NMR spectra were recorded for qualitative and quantitative analysis on native high-molecular-weight succinoglycans (both in the Na+ salt and free-acid forms) from Pseudomonas sp. NCIB 11592, Agrobacterium radiobacter A201-25, Rhizobium meliloti YE-2, and Rhizobium sp. isolated from Vicia faba and compared with those of the deacylated and deacylated-depyruvated, partially depolymerised exopolysaccharides from Rhizobium meliloti YE-2. Moreover, a series of two-dimensional experiments was performed on all the exopolysaccharides aiming at the partial assignment of the NMR spectra. The NMR data showed that succinate is located on O-6 of either one or both of the two side chain 3-linked β-D-Glc residues, whereas the acetate (when it is present) is located on one of the O-6 of backbone 4-linked β-D-Glc units, but the specific site could not be determined. In addition, the spectral features of the succinate substituent were found to be sensitive to pH changes.

  NMR analysis, Succinoglycans, an NMR analysis of

  NCBI PubMed ID: 7842440
  Publication DOI: 10.1016/0008-6215(94)00227-4
  Journal NLM ID: 0043535
  Publisher: Elsevier
  Institutions: Centro Ricerche POLY-biós LBT-Area di Ricerca, Padriciano 99, I-34012 Trieste, Italy, Dipartimento di Biochimica, Biofisica e Chimica delle Macromolecole, Università degli Studi di Trieste, Via L. Giorgieri 1, I-34127 Trieste, Italy
  Methods: 13C NMR, 1H NMR, NMR-2D, deacylation
  
   
  
  Agrobacterium radiobacter A201-25, Pseudomonas sp. NCIB11592, Rhizobium, Rhizobium meliloti YE-2
  CSDB ID 118260 (all data & tools)
• Article ID: 6412
  Kelly SD, Ovchinnikova OG, Muller F, Steffen M, Braun M, Sweeney RP, Kowarik M, Follador R, Lowary TL, Serventi F, Whitfield C "Identification of a second glycoform of the clinically prevalent O1 antigen from Klebsiella pneumoniae" - Proceedings of the National Academy of Sciences of the USA 120(29) (2023) e2301302120
  

  Carbapenemase and extended beta-lactamase-producing Klebsiella pneumoniae isolates represent a major health threat, stimulating increasing interest in immunotherapeutic approaches for combating Klebsiella infections. Lipopolysaccharide O antigen polysaccharides offer viable targets for immunotherapeutic development, and several studies have described protection with O-specific antibodies in animal models of infection. O1 antigen is produced by almost half of clinical Klebsiella isolates. The O1 polysaccharide backbone structure is known, but monoclonal antibodies raised against the O1 antigen showed varying reactivity against different isolates that could not be explained by the known structure. Reinvestigation of the structure by NMR spectroscopy revealed the presence of the reported polysaccharide backbone (glycoform O1a), as well as a previously unknown O1b glycoform composed of the O1a backbone modified with a terminal pyruvate group. The activity of the responsible pyruvyltransferase (WbbZ) was confirmed by western immunoblotting and in vitro chemoenzymatic synthesis of the O1b terminus. Bioinformatic data indicate that almost all O1 isolates possess genes required to produce both glycoforms. We describe the presence of O1ab-biosynthesis genes in other bacterial species and report a functional O1 locus on a bacteriophage genome. Homologs of wbbZ are widespread in genetic loci for the assembly of unrelated glycostructures in bacteria and yeast. In K. pneumoniae, simultaneous production of both O1 glycoforms is enabled by the lack of specificity of the ABC transporter that exports the nascent glycan, and the data reported here provide mechanistic understanding of the capacity for evolution of antigenic diversity within an important class of biomolecules produced by many bacteria.

  Lipopolysaccharide, O antigen, Klebsiella pneumoniae, antigenic diversity, vaccine candidate

  NCBI PubMed ID: 37428935
  Publication DOI: 10.1073/pnas.2301302120
  Journal NLM ID: 7505876
  Publisher: National Academy of Sciences
  Correspondence: F. Serventi ; C. Whitfield
  Institutions: Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON N1G 2W1, Canada, LimmaTech Biologics AG, Schlieren 8952, Switzerland, Department of Chemistry, University of Alberta, Edmonton, AB T6G 2G2, Canada, Institute of Biological Chemistry, Academia Sinica, Taipei, Nangang 11529, Taiwan, Institute of Biochemical Sciences, National Taiwan University, Taipei 10617, Taiwan
  Methods: 13C NMR, 1H NMR, NMR-2D, SDS-PAGE, ESI-MS, mild acid hydrolysis, Western blotting, genetic methods, enzyme assay, LC-MS, bioinformatic analysis, SEC, phylogenetic analysis, monoclonal antibodies
  
   
  
  Rhizobium meliloti
  CSDB ID 22775 (all data & tools)