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Chen J, Gao J, Ju J, Yang S
A hyaluronan-based polysaccharide peptide generated by a genetically modified Streptococcus zooepidemicus
Carbohydrate Research 478(1) (2019)
25-32
Streptococcus zooepidemicus ATCC 39920 (S-2)
(Ancestor NCBI TaxID 1301,
species name lookup)
Streptococcus zooepidemicus ATCC 39920 (S-3)
(Ancestor NCBI TaxID 1301,
species name lookup)
Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
The structure was elucidated in this paperPublication DOI: 10.1016/j.carres.2019.04.005Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: S. Yang <yshulin
njust.edu.cn>
Institutions: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Xiaolingwei 200, Nanjing, 210094, China
Polysaccharide peptides (or protein-bound polysaccharides, PSPs) are commonly found in mushrooms and plants and possess important nutritional properties and health benefits. The pathogenic bacterium Streptococcus zooepidemicus does not inherently produce PSPs but secretes the capsular polysaccharide hyaluronan. However, in a previous investigation of the catalytic mechanism of UDP-glucose dehydrogenase (UGDH), a PSP of peptide-bound hyaluronan was found to be produced by S. zooepidemicus through the in vivo expression of a mutant of the gene encoding UGDH. In the present study, this hyaluronan-derived PSP was structurally characterized by FT-IR, NMR, and high-performance liquid chromatography-mass spectrometry (HPLC-MS), and the data confirmed that the polysaccharide backbone, hyaluronan, is covalently bound to the side-chain peptides via an amide linkage. More importantly, the bacterial production of a PSP via this genetic modification method should inspire further research on the in vitro enzymatic synthesis of PSPs or even naturally occurring polysaccharide derivatives and may provide a theoretical foundation for investigating the in vivo synthetic mechanism of PSPs.
synthesis, Hyaluronan (HA), Polysaccharide peptide (PSP), Streptococcus zooepidemicus, UDP-Glucose dehydrogenase
Structure type: oligomer
Location inside paper: p.27, fig.4A, HAd
Trivial name: hyaluronan, polysaccharide peptide HAd
Compound class: PSP
Contained glycoepitopes: IEDB_115136,IEDB_135813,IEDB_137340,IEDB_140630,IEDB_141807,IEDB_151531,IEDB_423153
Methods: 13C NMR, 1H NMR, DNA techniques, acid hydrolysis, FTIR, genetic methods, HPSEC-MALLS, HPLC-MS, mutagenesis, proteinase K digestion
Comments, role: disaccharide unit of HA from the recombination strains S. zooepidemicus ATCC 3992 (S-2 and S-3), in which hasB* is incorporated into the genomic DNA and plasmid pKSV7-hasB*.
Related record ID(s): 660, 986
NCBI Taxonomy refs (TaxIDs): 1301Reference(s) to other database(s): GTC:G86299UP, GlycomeDB:
19498
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There is only one chemically distinct structure:
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Chen J, Gao J, Ju J, Yang S
A hyaluronan-based polysaccharide peptide generated by a genetically modified Streptococcus zooepidemicus
Carbohydrate Research 478(1) (2019)
25-32
Streptococcus zooepidemicus ATCC 39920 (S-3)
(Ancestor NCBI TaxID 1301,
species name lookup)
Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
The structure was elucidated in this paperPublication DOI: 10.1016/j.carres.2019.04.005Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: S. Yang <yshulin
njust.edu.cn>
Institutions: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Xiaolingwei 200, Nanjing, 210094, China
Polysaccharide peptides (or protein-bound polysaccharides, PSPs) are commonly found in mushrooms and plants and possess important nutritional properties and health benefits. The pathogenic bacterium Streptococcus zooepidemicus does not inherently produce PSPs but secretes the capsular polysaccharide hyaluronan. However, in a previous investigation of the catalytic mechanism of UDP-glucose dehydrogenase (UGDH), a PSP of peptide-bound hyaluronan was found to be produced by S. zooepidemicus through the in vivo expression of a mutant of the gene encoding UGDH. In the present study, this hyaluronan-derived PSP was structurally characterized by FT-IR, NMR, and high-performance liquid chromatography-mass spectrometry (HPLC-MS), and the data confirmed that the polysaccharide backbone, hyaluronan, is covalently bound to the side-chain peptides via an amide linkage. More importantly, the bacterial production of a PSP via this genetic modification method should inspire further research on the in vitro enzymatic synthesis of PSPs or even naturally occurring polysaccharide derivatives and may provide a theoretical foundation for investigating the in vivo synthetic mechanism of PSPs.
synthesis, Hyaluronan (HA), Polysaccharide peptide (PSP), Streptococcus zooepidemicus, UDP-Glucose dehydrogenase
Structure type: oligomer
Location inside paper: p.27, fig.4B, HAd
Trivial name: polysaccharide peptide HAd
Compound class: PSP
Contained glycoepitopes: IEDB_115136,IEDB_135813,IEDB_137340,IEDB_140630,IEDB_141807,IEDB_151531,IEDB_423153
Methods: 13C NMR, 1H NMR, DNA techniques, acid hydrolysis, FTIR, genetic methods, HPSEC-MALLS, HPLC-MS, mutagenesis, proteinase K digestion
Comments, role: disaccharide unit of HAd from the mutant strain S. zooepidemicus ATCC 3992 (hasB* encoding the Cys260Ala mutant).
Related record ID(s): 659, 986
NCBI Taxonomy refs (TaxIDs): 1301
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There is only one chemically distinct structure:
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Chen J, Gao J, Ju J, Yang S
A hyaluronan-based polysaccharide peptide generated by a genetically modified Streptococcus zooepidemicus
Carbohydrate Research 478(1) (2019)
25-32
|
Subst-(1-6)-+
|
-4)-b-D-GlcpA-(1-3)-b-D-GlcpNAc-(1-4)-b-D-GlcpA-(1-3)-b-D-GlcpN-(1-
Subst = peptide |
Show graphically |
Streptococcus zooepidemicus ATCC 39920 (S-3)
(Ancestor NCBI TaxID 1301,
species name lookup)
Taxonomic group: bacteria / Firmicutes
(Phylum: Firmicutes)
The structure was elucidated in this paperPublication DOI: 10.1016/j.carres.2019.04.005Journal NLM ID: 0043535Publisher: Elsevier
Correspondence: S. Yang <yshulin
njust.edu.cn>
Institutions: School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Xiaolingwei 200, Nanjing, 210094, China
Polysaccharide peptides (or protein-bound polysaccharides, PSPs) are commonly found in mushrooms and plants and possess important nutritional properties and health benefits. The pathogenic bacterium Streptococcus zooepidemicus does not inherently produce PSPs but secretes the capsular polysaccharide hyaluronan. However, in a previous investigation of the catalytic mechanism of UDP-glucose dehydrogenase (UGDH), a PSP of peptide-bound hyaluronan was found to be produced by S. zooepidemicus through the in vivo expression of a mutant of the gene encoding UGDH. In the present study, this hyaluronan-derived PSP was structurally characterized by FT-IR, NMR, and high-performance liquid chromatography-mass spectrometry (HPLC-MS), and the data confirmed that the polysaccharide backbone, hyaluronan, is covalently bound to the side-chain peptides via an amide linkage. More importantly, the bacterial production of a PSP via this genetic modification method should inspire further research on the in vitro enzymatic synthesis of PSPs or even naturally occurring polysaccharide derivatives and may provide a theoretical foundation for investigating the in vivo synthetic mechanism of PSPs.
synthesis, Hyaluronan (HA), Polysaccharide peptide (PSP), Streptococcus zooepidemicus, UDP-Glucose dehydrogenase
Structure type: polymer chemical repeating unit
Location inside paper: p.26, fig.1, HAd
Trivial name: polysaccharide peptide HAd
Compound class: PSP
Contained glycoepitopes: IEDB_115136,IEDB_135813,IEDB_137340,IEDB_140630,IEDB_141807,IEDB_151527,IEDB_151531,IEDB_423153
Methods: 13C NMR, 1H NMR, DNA techniques, acid hydrolysis, FTIR, genetic methods, HPSEC-MALLS, HPLC-MS, mutagenesis, proteinase K digestion
Comments, role: proposed structure HAd in the recombination strain (S-3) from S. zooepidemicus ATCC 39920, in which hasB* is incorporated into the genomic DNA and plasmid pKSV7-hasB*.
Related record ID(s): 659, 660
NCBI Taxonomy refs (TaxIDs): 1301
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There is only one chemically distinct structure:
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