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Chi Y, Ye H, Li H, Li Y, Guan H, Mou H, Wang P
Structure and molecular morphology of a novel moisturizing exopolysaccharide produced by Phyllobacterium sp. 921F
International Journal of Biological Macromolecules 135 (2019)
1034-1042
|
b-D-Glcp-(1-3)-+ S-Pyr-(2-6:2-4)-+
| |
S-Pyr-(2-6:2-4)-a-D-Galp-(1-4)-b-D-Glcp-(1-3)-a-D-Galp |
Show graphically |
Phyllobacterium sp. 921F
(Ancestor NCBI TaxID 1871046,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 31173824Publication DOI: 10.1016/j.ijbiomac.2019.06.019.Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: H. Mou <mousun
ouc.edu.cn>; P. Wang <pengwang
ouc.edu.cn>
Institutions: School of Medicine and Pharmacy, Ocean University of China, Qingdao, China, College of Food Science and Engineering, Ocean University of China, Qingdao, China
Bacterial exopolysaccharides (EPSs) are widely applied in food, cosmetic, and medical industries. The EPS produced by Phyllobacterium sp. 921F was a novel polysaccharide, which exhibits attractive characteristics of high yield, favorable rheological properties, and excellent moisture retention ability. Considering the complexity of polysaccharide structures, specific enzymatic hydrolysis was employed here to resolve the structure of the EPS. End-products including tetra-, hexa- and octa-saccharides were isolated. According to their mass spectroscopy (MS) and nuclear magnetic resonance (NMR) spectra, the backbone of the EPS was found to be mainly comprising a →4)-β-d-Glcp-(1→3)-α-d-Galp(4,6-S-Pyr)-(1→ disaccharide repeating units. Based on atomic force microscopy results, EPS exhibited characteristics that were consistent with a stiff, elongated molecule with no branches. The length and height of the single molecular chain were approximately 600 and 0.7 nm, respectively. Our clarification of structure and molecular morphology of EPS from Phyllobacterium sp. 921F provide a foundation for the industrial application of this potential moisture-retaining material.
exopolysaccharide, Structural characterization, molecular morphology
Structure type: oligomer ; 806
Location inside paper: p.1002, table 1, fig.6a, P1
Compound class: EPS
Contained glycoepitopes: IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_151528,IEDB_190606,IEDB_983931,SB_192,SB_7
Methods: 13C NMR, 1H NMR, gel filtration, NMR-2D, enzymatic hydrolysis, FTIR, atomic force microscopy, ESI-CID-MS, reduction, ESI-CID-MS/MS, molecular morphology
Comments, role: End-products (fraction P1) of the enzymatic hydrolysis of the EPS from Phyllobacterium sp. 921F.; published 13С chemical shifts contradict to the structure: Gal-I C2 (77.1), Gal-I C3 (67.0), Glcp-I C3 (70.1), Galp-II C2 (77.1), Glcp-II C3 (69.6). NMRC: #3,4,3_bDGlcp 104.0 72.1 69.6 75.8 73.2 60.5 // #3,4,6_xSPyr 175.7 100.5 25.1 // #3,4_aDGalp 99.5 77.1 76.5 62.5 72.0 65.0 // #3_bDGlcp 104.3 72.0 70.1 81.2 75.5 60.7 // #6_xSPyr 175.7 100.5 25.1 // #_aDGalp 92.6 77.1 67.0 72.0 62.1 65.1 //.
Related record ID(s): 662, 663, 988
NCBI Taxonomy refs (TaxIDs): 1871046
Show glycosyltransferases
NMR conditions: in D2O at 293 K
[as TSV]
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,3 bDGlcp 4.69 3.42 3.42 3.50 3.41 3.72-3.88
3,4,6 xSPyr - - 1.46
3,4 aDGalp 5.43 4.10 4.16 4.16 4.44 3.85-4.02
3 bDGlcp 4.67 3.48 3.64 3.66 3.43 3.72-3.88
6 xSPyr - - 1.46
aDGalp 5.31 4.07 4.07 4.45 3.90 3.85-4.02
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4,3 | bDGlcp | 4.69 | 3.42 | 3.42 | 3.50 | 3.41 | 3.72
3.88 |
| 3,4,6 | xSPyr |
|
| 1.46 | |
| 3,4 | aDGalp | 5.43 | 4.10 | 4.16 | 4.16 | 4.44 | 3.85
4.02 |
| 3 | bDGlcp | 4.67 | 3.48 | 3.64 | 3.66 | 3.43 | 3.72
3.88 |
| 6 | xSPyr |
|
| 1.46 | |
| | aDGalp | 5.31 | 4.07 | 4.07 | 4.45 | 3.90 | 3.85
4.02 |
|
13C NMR data: present in publication
|
There is only one chemically distinct structure:
Expand this record
Collapse this record
Chi Y, Ye H, Li H, Li Y, Guan H, Mou H, Wang P
Structure and molecular morphology of a novel moisturizing exopolysaccharide produced by Phyllobacterium sp. 921F
International Journal of Biological Macromolecules 135 (2019)
1034-1042
|
b-D-Glcp-(1-3)-+ S-Pyr-(2-6:2-4)-+
| |
S-Pyr-(2-6:2-4)-a-D-Galp-(1-4)-b-D-Glcp-(1-3)-b-D-Galp |
Show graphically |
Phyllobacterium sp. 921F
(Ancestor NCBI TaxID 1871046,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 31173824Publication DOI: 10.1016/j.ijbiomac.2019.06.019.Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: H. Mou <mousun
ouc.edu.cn>; P. Wang <pengwang
ouc.edu.cn>
Institutions: School of Medicine and Pharmacy, Ocean University of China, Qingdao, China, College of Food Science and Engineering, Ocean University of China, Qingdao, China
Bacterial exopolysaccharides (EPSs) are widely applied in food, cosmetic, and medical industries. The EPS produced by Phyllobacterium sp. 921F was a novel polysaccharide, which exhibits attractive characteristics of high yield, favorable rheological properties, and excellent moisture retention ability. Considering the complexity of polysaccharide structures, specific enzymatic hydrolysis was employed here to resolve the structure of the EPS. End-products including tetra-, hexa- and octa-saccharides were isolated. According to their mass spectroscopy (MS) and nuclear magnetic resonance (NMR) spectra, the backbone of the EPS was found to be mainly comprising a →4)-β-d-Glcp-(1→3)-α-d-Galp(4,6-S-Pyr)-(1→ disaccharide repeating units. Based on atomic force microscopy results, EPS exhibited characteristics that were consistent with a stiff, elongated molecule with no branches. The length and height of the single molecular chain were approximately 600 and 0.7 nm, respectively. Our clarification of structure and molecular morphology of EPS from Phyllobacterium sp. 921F provide a foundation for the industrial application of this potential moisture-retaining material.
exopolysaccharide, Structural characterization, molecular morphology
Structure type: oligomer ; 806
Location inside paper: p.1002, table 1, fig.6a, P1
Compound class: EPS
Contained glycoepitopes: IEDB_136044,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_151528,IEDB_190606,IEDB_983931,SB_165,SB_166,SB_187,SB_192,SB_195,SB_7,SB_88
Methods: 13C NMR, 1H NMR, gel filtration, NMR-2D, enzymatic hydrolysis, FTIR, atomic force microscopy, ESI-CID-MS, reduction, ESI-CID-MS/MS, molecular morphology
Comments, role: End-products (fraction P1) of the enzymatic hydrolysis of the EPS from Phyllobacterium sp. 921F.; published 13С chemical shifts contradict to the structure: Glcp-I C3 (70.1), Galp-II C2 (77.1), Glcp-II C3 (69.6). NMRC: #3,4,3_bDGlcp 104.0 72.1 69.6 75.8 73.2 60.5 // #3,4,6_xSPyr 175.7 100.5 25.1 // #3,4_aDGalp 99.5 77.1 76.5 62.5 72.0 65.0 // #3_bDGlcp 104.3 72.0 70.1 81.2 75.5 60.7 // #6_xSPyr 175.7 100.5 25.1 // #_bDGalp 95.8 70.4 80.3 72.0 65.9 65.1 //.
Related record ID(s): 661, 663, 988
NCBI Taxonomy refs (TaxIDs): 1871046
Show glycosyltransferases
NMR conditions: in D2O at 293 K
[as TSV]
1H NMR data:
Linkage Residue H1 H2 H3 H4 H5 H6
3,4,3 bDGlcp 4.69 3.42 3.42 3.50 3.41 3.72-3.88
3,4,6 xSPyr - - 1.46
3,4 aDGalp 5.43 4.10 4.16 4.16 4.44 3.85-4.02
3 bDGlcp 4.67 3.48 3.64 3.66 3.43 3.72-3.88
6 xSPyr - - 1.45
bDGalp 4.66 3.74 3.86 4.38 3.60 3.85-4.02
1H NMR data:
| Linkage | Residue | H1 | H2 | H3 | H4 | H5 | H6 |
|---|
| 3,4,3 | bDGlcp | 4.69 | 3.42 | 3.42 | 3.50 | 3.41 | 3.72
3.88 |
| 3,4,6 | xSPyr |
|
| 1.46 | |
| 3,4 | aDGalp | 5.43 | 4.10 | 4.16 | 4.16 | 4.44 | 3.85
4.02 |
| 3 | bDGlcp | 4.67 | 3.48 | 3.64 | 3.66 | 3.43 | 3.72
3.88 |
| 6 | xSPyr |
|
| 1.45 | |
| | bDGalp | 4.66 | 3.74 | 3.86 | 4.38 | 3.60 | 3.85
4.02 |
|
13C NMR data: present in publication
|
There is only one chemically distinct structure:
Expand this record
Collapse this record
Chi Y, Ye H, Li H, Li Y, Guan H, Mou H, Wang P
Structure and molecular morphology of a novel moisturizing exopolysaccharide produced by Phyllobacterium sp. 921F
International Journal of Biological Macromolecules 135 (2019)
1034-1042
|
b-D-Glcp-(1-3)-+ S-Pyr-(2-6:2-4)-+ S-Pyr-(2-6:2-4)-+
| | |
S-Pyr-(2-6:2-4)-a-D-Galp-(1-4)-b-D-Glcp-(1-3)-a-D-Galp-(1-4)-b-D-Glcp-(1-3)-a-D-Galp |
Show graphically |
Phyllobacterium sp. 921F
(Ancestor NCBI TaxID 1871046,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 31173824Publication DOI: 10.1016/j.ijbiomac.2019.06.019.Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: H. Mou <mousun
ouc.edu.cn>; P. Wang <pengwang
ouc.edu.cn>
Institutions: School of Medicine and Pharmacy, Ocean University of China, Qingdao, China, College of Food Science and Engineering, Ocean University of China, Qingdao, China
Bacterial exopolysaccharides (EPSs) are widely applied in food, cosmetic, and medical industries. The EPS produced by Phyllobacterium sp. 921F was a novel polysaccharide, which exhibits attractive characteristics of high yield, favorable rheological properties, and excellent moisture retention ability. Considering the complexity of polysaccharide structures, specific enzymatic hydrolysis was employed here to resolve the structure of the EPS. End-products including tetra-, hexa- and octa-saccharides were isolated. According to their mass spectroscopy (MS) and nuclear magnetic resonance (NMR) spectra, the backbone of the EPS was found to be mainly comprising a →4)-β-d-Glcp-(1→3)-α-d-Galp(4,6-S-Pyr)-(1→ disaccharide repeating units. Based on atomic force microscopy results, EPS exhibited characteristics that were consistent with a stiff, elongated molecule with no branches. The length and height of the single molecular chain were approximately 600 and 0.7 nm, respectively. Our clarification of structure and molecular morphology of EPS from Phyllobacterium sp. 921F provide a foundation for the industrial application of this potential moisture-retaining material.
exopolysaccharide, Structural characterization, molecular morphology
Structure type: oligomer ; 806
Location inside paper: p.1002, fig.6b, p. 1004, P2
Compound class: EPS
Contained glycoepitopes: IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_151528,IEDB_190606,IEDB_983931,SB_192,SB_7
Methods: 13C NMR, 1H NMR, gel filtration, NMR-2D, enzymatic hydrolysis, FTIR, atomic force microscopy, ESI-CID-MS, reduction, ESI-CID-MS/MS, molecular morphology
Comments, role: End-products (fraction P2) of the enzymatic hydrolysis of the EPS from Phyllobacterium sp. 921F.
Related record ID(s): 661, 662, 988
NCBI Taxonomy refs (TaxIDs): 1871046
Show glycosyltransferases
There is only one chemically distinct structure:
Expand this record
Collapse this record
Chi Y, Ye H, Li H, Li Y, Guan H, Mou H, Wang P
Structure and molecular morphology of a novel moisturizing exopolysaccharide produced by Phyllobacterium sp. 921F
International Journal of Biological Macromolecules 135 (2019)
1034-1042
Phyllobacterium sp. 921F
(Ancestor NCBI TaxID 1871046,
species name lookup)
Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
The structure was elucidated in this paperNCBI PubMed ID: 31173824Publication DOI: 10.1016/j.ijbiomac.2019.06.019.Journal NLM ID: 7909578Publisher: Butterworth-Heinemann
Correspondence: H. Mou <mousun
ouc.edu.cn>; P. Wang <pengwang
ouc.edu.cn>
Institutions: School of Medicine and Pharmacy, Ocean University of China, Qingdao, China, College of Food Science and Engineering, Ocean University of China, Qingdao, China
Bacterial exopolysaccharides (EPSs) are widely applied in food, cosmetic, and medical industries. The EPS produced by Phyllobacterium sp. 921F was a novel polysaccharide, which exhibits attractive characteristics of high yield, favorable rheological properties, and excellent moisture retention ability. Considering the complexity of polysaccharide structures, specific enzymatic hydrolysis was employed here to resolve the structure of the EPS. End-products including tetra-, hexa- and octa-saccharides were isolated. According to their mass spectroscopy (MS) and nuclear magnetic resonance (NMR) spectra, the backbone of the EPS was found to be mainly comprising a →4)-β-d-Glcp-(1→3)-α-d-Galp(4,6-S-Pyr)-(1→ disaccharide repeating units. Based on atomic force microscopy results, EPS exhibited characteristics that were consistent with a stiff, elongated molecule with no branches. The length and height of the single molecular chain were approximately 600 and 0.7 nm, respectively. Our clarification of structure and molecular morphology of EPS from Phyllobacterium sp. 921F provide a foundation for the industrial application of this potential moisture-retaining material.
exopolysaccharide, Structural characterization, molecular morphology
Structure type: polymer chemical repeating unit
Location inside paper: abstract, p.1004
Compound class: EPS
Contained glycoepitopes: IEDB_136906,IEDB_137472,IEDB_141794,IEDB_142488,IEDB_146664,IEDB_151528,IEDB_190606,IEDB_983931,SB_192,SB_7
Methods: 13C NMR, 1H NMR, gel filtration, NMR-2D, enzymatic hydrolysis, FTIR, atomic force microscopy, ESI-CID-MS, reduction, ESI-CID-MS/MS, molecular morphology
Related record ID(s): 661, 662, 663
NCBI Taxonomy refs (TaxIDs): 1871046
Show glycosyltransferases
There is only one chemically distinct structure:
Expand this record
Collapse this record
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