Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: root
NCBI PubMed ID: 10718627Publication DOI: 10.1023/a:1007028320041Journal NLM ID: 0364456Publisher: New York: Springer
Institutions: Department of Pharmacology, Faculty of Medicine, The University of Hong Kong, Hong Kong, China, Department of Pharmacology, Norman Bethune University of Medical Sciences, Changchun, China, Department of Clinical Pharmacology, Sun Yat-sen University of Medical Sciences, Guangzhou, China
Inasmuch as the oxidation of low-density lipoprotein (Ox-LDL) may play a key role in the initiation and progression of atherosclerosis, it has become increasingly important to identify potential antioxidants. Panax quinquefolium saponins (PQS) are extracted from the stems and leaves of the North American form of ginseng,Panax quinquefolium. Our previous studies have indicated that PQS (0.25-1 mg/ml) can protect against oxidation of LDLin vitro. The purpose of the current work was to investigate the potential interaction of lower concentrations of PQS (1-100 μg/ml) with vitamin C on the reduction of LDL oxidation. LDL was isolated from the plasma of healthy human donors by sequential ultracentrifugation. Native LDL (0.05 or 0.2 mg/ml) was incubated with PQS and/or vitamin C for 30 min at 20°C. Oxidative modification was initiated with 2 μM or 5 μM CuSO4 at 37°C for 0-24 h. Pretreatment with PQS (100 μg/ml) reduced alterations in phospholipids, lipid peroxide levels and relative electrophoretic mobility of Ox-LDL. The presence of vitamin C (1-10 μM) significantly enhanced the protective effects of PQS. Pretreatment with PQS (1-100 μg/ml) resulted in concentration-dependent inhibition of LDL oxidation and prolongation of lag time as determined from measurements of conjugated lipid hydroperoxide content in Ox-LDL samples. Interestingly, the inhibitory actions of lower amounts of PQS (1 and 10 μg/ml) on the formation of conjugated dienes were significantly increased when vitamin C (0.1 or 1 μM) was present. In conclusion, our results suggest that PQS not only have direct antioxidant property but at low concentrations, their actions can be enhanced by vitamin C.
Antioxidant, Panax quinquefolium saponins, vitamin C, LDL oxidation
Structure type: monomer
Location inside paper: p. 78, left column, paragraph 3
Trivial name: ginsenoside Rh2, (20S)-ginsenoside Rh2, ginsenoside
Compound class: saponin glycoside, glycoside, triterpenoid glycoside
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Related record ID(s): 66315, 66316, 66317, 66318, 66319, 66320, 66321, 66323
NCBI Taxonomy refs (TaxIDs): 44588
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There is only one chemically distinct structure:
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(later renamed to: Panax quinquefolius)