Extraction of Evernia prunastri with hot aqueous alkali solubilized heteropolysaccharide-containing material which was purified via Fehling precipitation. Further workup of this polysaccharide with Cetavlon gave two fractions having related but different structures. Each structure consisted of a 1→6-linked α-D-man p main chain partially monosubstituted at O-2 with side chains of α-D-Galp and partially disubstituted at O-2 and O-4 with α-D-Galp and β-D-Galp, respectively. The fractions differed in that one contained much more uronic acid than the other, with corresponding predominance of side chains containing α-D-GlcpA-(1→3)-D-Glcp, α-D-Galp-(1→2)-D-Glcp, and β-D-Gal f units.
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