Aussel L, Chaby R, Le Blay K, Kelly J, Thibault PE, Perry MB, Caroff M Chemical and serological characterization of the Bordetella hinzii lipopolysaccharides FEBS Letters485(1) (2000)
40-46
The structure was elucidated in this paper NCBI PubMed ID:11086162 Journal NLM ID:0155157 Publisher: Elsevier Correspondence: martine.caroffbbmpc.u-psud.fr Institutions: Equipe `Endotoxines', UMR 8619 du Centre National de la Recherche Scientifique, Biochimie, Universite de Paris-Sud, F-Orsay, France, Institute for Biological Sciences, National Research Council of Canada, Ottawa, Ont., Canada
Abstract Bordetella hinzii has recently been isolated from immunocompromised human hosts. The polysaccharides isolated from its endotoxin (lipopolysaccharide, LPS) were investigated using chemical analyses, NMR, gas-liquid chromatography/mass spectrometry and mass spectrometry by plasma desorption, matrix-assisted laser desorption/ionization and electrospray. The following structure for the O-chain-free LPS was deduced from the experimental results : . Mass spectrometry and serology revealed that the O-chains were different from the homopolymer common to Bordetella bronchiseptica and Bordetella parapertussis strains and were composed of a trisaccharide repeating unit. Masses up to 8 kDa were obtained for native LPS molecular species.
Lipopolysaccharide, structure, endotoxin, serology, mass spectrometry, Bordetella hinzii
El Hamidi A, Tirsoaga A, Novikov A, Hussein A, Caroff M Microextraction of bacterial lipid A: easy and rapid method for mass spectrometric characterization Journal of Lipid Research46(8) (2005)
1773-1778
NCBI PubMed ID:15930524 Journal NLM ID:0376606 Publisher: ASBMB Correspondence: martine.caroffbbmpc.u-psud.fr Institutions: Equipe Endotoxines, Unité Mixte de Recherche 8619 du Centre National de la Recherche Scientifique, Institut de Biochimie, Biophysique Moléculaire et Cellulaire, Université de Paris-Sud, Orsay, France, Department of Physical Chemistry, University of Bucharest, Bucharest, Romania
Endotoxins (lipopolysaccharides) are the main components of Gram-negative bacterial outer membranes. A quick and simple way to isolate their lipid region (lipid A) directly from whole bacterial cells was devised. This method using hot ammonium-isobutyrate solvent was applied to small quantities of cells and proved to be indispensable when a rapid characterization of lipid A structure by mass spectrometry was required. Biological activities of endotoxins are directly related to the lipid A structures, which vary greatly with cell growth conditions. This method is suitable for rough- and smooth-type bacteria and very efficient for screening variations in lipid A structures. Data are acquired in a few hours and avoid the use of phenol in extraction.
El Hamidi A, Tirsoaga A, Novikov A, Hussein A, Caroff M Microextraction of bacterial lipid A: easy and rapid method for mass spectrometric characterization Journal of Lipid Research46(8) (2005)
1773-1778
NCBI PubMed ID:15930524 Journal NLM ID:0376606 Publisher: ASBMB Correspondence: martine.caroffbbmpc.u-psud.fr Institutions: Equipe Endotoxines, Unité Mixte de Recherche 8619 du Centre National de la Recherche Scientifique, Institut de Biochimie, Biophysique Moléculaire et Cellulaire, Université de Paris-Sud, Orsay, France, Department of Physical Chemistry, University of Bucharest, Bucharest, Romania
Endotoxins (lipopolysaccharides) are the main components of Gram-negative bacterial outer membranes. A quick and simple way to isolate their lipid region (lipid A) directly from whole bacterial cells was devised. This method using hot ammonium-isobutyrate solvent was applied to small quantities of cells and proved to be indispensable when a rapid characterization of lipid A structure by mass spectrometry was required. Biological activities of endotoxins are directly related to the lipid A structures, which vary greatly with cell growth conditions. This method is suitable for rough- and smooth-type bacteria and very efficient for screening variations in lipid A structures. Data are acquired in a few hours and avoid the use of phenol in extraction.
El Hamidi A, Tirsoaga A, Novikov A, Hussein A, Caroff M Microextraction of bacterial lipid A: easy and rapid method for mass spectrometric characterization Journal of Lipid Research46(8) (2005)
1773-1778
NCBI PubMed ID:15930524 Journal NLM ID:0376606 Publisher: ASBMB Correspondence: martine.caroffbbmpc.u-psud.fr Institutions: Equipe Endotoxines, Unité Mixte de Recherche 8619 du Centre National de la Recherche Scientifique, Institut de Biochimie, Biophysique Moléculaire et Cellulaire, Université de Paris-Sud, Orsay, France, Department of Physical Chemistry, University of Bucharest, Bucharest, Romania
Endotoxins (lipopolysaccharides) are the main components of Gram-negative bacterial outer membranes. A quick and simple way to isolate their lipid region (lipid A) directly from whole bacterial cells was devised. This method using hot ammonium-isobutyrate solvent was applied to small quantities of cells and proved to be indispensable when a rapid characterization of lipid A structure by mass spectrometry was required. Biological activities of endotoxins are directly related to the lipid A structures, which vary greatly with cell growth conditions. This method is suitable for rough- and smooth-type bacteria and very efficient for screening variations in lipid A structures. Data are acquired in a few hours and avoid the use of phenol in extraction.
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