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1. (Article ID: 6706)
 
Yoshioka Y, Tabeta R, Saito H, Uehara N, Fukuoka F
Antitumor polysaccharides from P. ostreatus (FR.) Quel.: Isolation and structure of a b-Glucan
Carbohydrate Research 140 (1985) 93-100
 

We isolated an antitumor glucan (HA β-glucan) from the neutral polysaccharide fraction (A3) of a hot-water extract of the edible mushroom P. ostreatus (Fr.) Quél. Purification was accomplished by extractions with 20% sodium chloride solution saturated with thymol and by precipitations with ethanol from dimethyl sulfoxide solution. The glucan showed marked antitumor activity at a dose of 0.1 mg/kg. It is a highly branched (1----3)-β-glucan having an average structure represented by a pentasaccharide segment consisting of one nonreducing terminal, one 3,6-di-O-substituted, and three 3-mono-O-substituted β-D-glucopyranosyl residues. This structure was confirmed by examining 13C-n.m.r. spectra taken at 75.46 MHz.

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