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Taxonomic group: plant / Streptophyta (Phylum: Streptophyta)
Organ / tissue: fruit

NCBI PubMed ID: 10898610
Publication DOI: 10.1021/jf990924+
Journal NLM ID: 0374755
Publisher: American Chemical Society
Correspondence: olle

cirad.fr
Institutions: Centre de Coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), Départment FLHOR, Montpellier, France, Institut National de la Recherche Agronomique (INRA), Station de Recherches Cidricoles et de Biotransformation des Fruits et Légumes, Le Rheu, France

Ripe mango puree (Smith cultivar) was treated with fungal polysaccharidases containing pectinolytic, hemicellulolytic, and cellulolytic activities for 2 h at 50 °C. A loss of 30% of the cell wall material (CWM) was measured. CWM polysaccharides were hydrolyzed to varying degrees: 88, 65, and 65% of, respectively, galacturonic acid-, arabinose-, and rhamnose-containing polymers were hydrolyzed, whereas 50% of cellulose was degraded. After 30 min of treatment, the ethanol precipitation test on the serum was negative, indicating that pectic substances were rapidly hydrolyzed. Oligogalacturonic acids (degree of polymerization, 1−12) were observed in the serum. A viscosity drop of 90% was measured after 2 h, confirming the dominant role of pectic substances in puree viscosity.

mango; Mangifera indica L.; cell wall polysaccharides; enzymatic treatment; viscosity

Structure type: oligomer
Location inside paper: Fig. 1, DP2-12
Compound class: oligosaccharide, galacturonan

Methods: acid hydrolysis, GC, enzymatic digestion, HPAEC-PAD, acetylation, reduction, Kjeldahl method, viscosimetry
Enzymes that release or process the structure: endopolygalacturonase

NCBI Taxonomy refs (TaxIDs): 29780
Show glycosyltransferases

There is only one chemically distinct structure:

O=C(O)[C@H]1O[C@H](O[C@H]2[C@@H](C(=O)O)O[C@H](O)[C@H](O)[C@H]2O)[C@H](O)[C@@H](O)[C@H]1O

SVG MW SMILES 3D mol Ionize

Warning = sub-repeat ending at aDGalpA: variable unit count (1-11) was assumed to be 1