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1. (Article ID: 6740)
 
Kapteyn JC, Montijn RC, Dijkgraaf GJP, van den Ende H, Klis FM
Covalent association of b-1,3-glucan with b-1,6-glucosylated mannoproteins in cell walls of Candida albicans
Journal of Bacteriology 177 (1995) 3788-3792
 

Yeast and hyphal walls of Candida albicans were extracted with sodium dodecyl sulfate (SDS). Some of the extracted proteins reacted with a specific β-1,6-glucan antiserum but not with a β-1,3-glucan antiserum. They lost their β-1,6-glucan epitope after treatment with ice-cold aqueous hydrofluoric acid, suggesting that β-1,6-glucan was linked to the protein through a phosphodiester bridge. When yeast and hyphal walls extracted with SDS were subsequently extracted with a pure β-1,3-glucanase, several mannoproteins that were recognized by both the β-1,6-glucan antiserum and the β-1,3-glucan antiserum were released. Both epitopes were sensitive to aqueous hydrofluoric acid treatment, suggesting that β-1,3-glucan and β-1,6-glucan are linked to proteins by phosphodiester linkages. The possible role of β-glucans in the retention of cell wall proteins is discussed.

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