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Reynoird JP, Dewitte W, Prinsen E, Van Onckelen H, Noin M, Chriqui D
Shooty tumours induced on Gerbera hybrida leaf explants by A. tumefaciens strain 82139 are characterized by high endogenous cytokinin levels
Acta Horticulture 508 (2000)
261-263
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b-D-Ribf-(1-7)-Subst
Subst = trans-zeatin = SMILES C/C(=C\CNC1=NC=NC2=C1{7}NC=N2)/{13}CO |
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Gerbera hybrida
(later renamed to: Gerbera hybrid cultivar)
(NCBI TaxID 18101,
species name lookup)
Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: leaf
Publication DOI: 10.17660/ActaHortic.2000.508.41Institutions: INRA C.R Angers, Unité d' Amélioration des Espèces Frutières et Ornementales, Beaucouzé, France, University of Antwerp (UIA), Department of Biology, Antwerp, Belgium, Université P. et M. Curie, Laboratoire C.E.M.V., Paris, France
Genetic transformation depends upon the regeneration efficiency. In a precedent work[1], we found differences in bud regeneration capacity from G. hybrida leaf explants excised from various genotypes. In particular, clone 10 regenerated whereas clone 11 did not. Moreover, only juvenile leaf explants regenerated in the presence of BA and NAA. In order to enhance regeneration from mature Gerbera leaf explants, we investigated in this work effect of inoculation with A. tumefaciens strain 82139. As for other species [2,3], this strain was able to induce shooty tumours. This capacity was related to high levels of zeatin riboside as revealed by hormone quantification.
Agrobacterium, Auxin, LC-MS, in vitro culture, cytokinin, Gerbera, bud regeneration
Structure type: monomer
Location inside paper: p. 263, Fig. 1, ZR
Trivial name: zeatinriboside
Compound class: glycoside, cytokinin
Contained glycoepitopes: IEDB_149136
Methods: PCR, MS, extraction, histochemical assay
Related record ID(s): 67448, 67449
NCBI Taxonomy refs (TaxIDs): 18101
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There is only one chemically distinct structure:
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Reynoird JP, Dewitte W, Prinsen E, Van Onckelen H, Noin M, Chriqui D
Shooty tumours induced on Gerbera hybrida leaf explants by A. tumefaciens strain 82139 are characterized by high endogenous cytokinin levels
Acta Horticulture 508 (2000)
261-263
Gerbera hybrida
(later renamed to: Gerbera hybrid cultivar)
(NCBI TaxID 18101,
species name lookup)
Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: leaf
Publication DOI: 10.17660/ActaHortic.2000.508.41Institutions: INRA C.R Angers, Unité d' Amélioration des Espèces Frutières et Ornementales, Beaucouzé, France, University of Antwerp (UIA), Department of Biology, Antwerp, Belgium, Université P. et M. Curie, Laboratoire C.E.M.V., Paris, France
Genetic transformation depends upon the regeneration efficiency. In a precedent work[1], we found differences in bud regeneration capacity from G. hybrida leaf explants excised from various genotypes. In particular, clone 10 regenerated whereas clone 11 did not. Moreover, only juvenile leaf explants regenerated in the presence of BA and NAA. In order to enhance regeneration from mature Gerbera leaf explants, we investigated in this work effect of inoculation with A. tumefaciens strain 82139. As for other species [2,3], this strain was able to induce shooty tumours. This capacity was related to high levels of zeatin riboside as revealed by hormone quantification.
Agrobacterium, Auxin, LC-MS, in vitro culture, cytokinin, Gerbera, bud regeneration
Structure type: monomer
Location inside paper: p. 263, Fig. 1, ZOG
Compound class: glycoside
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: PCR, MS, extraction, histochemical assay
Related record ID(s): 67447, 67449
NCBI Taxonomy refs (TaxIDs): 18101
Show glycosyltransferases
There is only one chemically distinct structure:
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Reynoird JP, Dewitte W, Prinsen E, Van Onckelen H, Noin M, Chriqui D
Shooty tumours induced on Gerbera hybrida leaf explants by A. tumefaciens strain 82139 are characterized by high endogenous cytokinin levels
Acta Horticulture 508 (2000)
261-263
Gerbera hybrida
(later renamed to: Gerbera hybrid cultivar)
(NCBI TaxID 18101,
species name lookup)
Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: leaf
Publication DOI: 10.17660/ActaHortic.2000.508.41Institutions: INRA C.R Angers, Unité d' Amélioration des Espèces Frutières et Ornementales, Beaucouzé, France, University of Antwerp (UIA), Department of Biology, Antwerp, Belgium, Université P. et M. Curie, Laboratoire C.E.M.V., Paris, France
Genetic transformation depends upon the regeneration efficiency. In a precedent work[1], we found differences in bud regeneration capacity from G. hybrida leaf explants excised from various genotypes. In particular, clone 10 regenerated whereas clone 11 did not. Moreover, only juvenile leaf explants regenerated in the presence of BA and NAA. In order to enhance regeneration from mature Gerbera leaf explants, we investigated in this work effect of inoculation with A. tumefaciens strain 82139. As for other species [2,3], this strain was able to induce shooty tumours. This capacity was related to high levels of zeatin riboside as revealed by hormone quantification.
Agrobacterium, Auxin, LC-MS, in vitro culture, cytokinin, Gerbera, bud regeneration
Structure type: monomer
Location inside paper: p. 263, Fig. 1, ZNG
Compound class: glycoside
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: PCR, MS, extraction, histochemical assay
Related record ID(s): 67447, 67448
NCBI Taxonomy refs (TaxIDs): 18101
Show glycosyltransferases
There is only one chemically distinct structure:
Expand this record
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