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Székács A, Hegedűs G, Tóbiás I, Pogány M, Barna B
Immunoassays for plant cytokinins as tools for the assessment of environmental stress and disease resistance
Analytica Chimica Acta 421(2) (2000)
135-146
|
b-D-Ribf-(1-7)-Subst
Subst = N6-isopent-2-enyladenine = SMILES C/C(C)=C/CNC1=C2C(N=C{7}N2)=NC=N1 |
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Nicotiana tabacum (CaMVp35S ipt mutant)
(Ancestor NCBI TaxID 4097,
species name lookup)
Nicotiana tabacum cv. Petit Havana
(Ancestor NCBI TaxID 4097,
species name lookup)
Nicotiana tabacum cv. Samsun
(Ancestor NCBI TaxID 4097,
species name lookup)
Lycopersicon esculentum cv. Linia XXIV
(later renamed to: Solanum lycopersicum cv. Linia XXIV)
(Ancestor NCBI TaxID 4081,
species name lookup)
Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Publication DOI: 10.1016/S0003-2670(00)01046-1Journal NLM ID: 0370534Correspondence: aszek
nki.hu
Institutions: Plant Protection Institute, Hungarian Academy of Sciences, Budapest, Hungary
The level of cytokinin type hormones present in plant tissues, such as N6-(2-isopentenyl)-adenosine (IPA), N6-(2-isopentenyl)-adenosine (2-iP), trans-zeatin riboside (ZR) and trans-zeatin (Z) is a good indicator of the resistance of plants to abiotic environmental stresses and to necrotic pathogens. Hapten-homologous and hapten-heterologous competitive indirect enzyme-linked immunosorbent assays (ELISAs) were developed, allowing the use of minute amounts of plant extracts for cytokinin analysis. These assays were used for the detection of members of the cytokinin plant hormone family including IPA and ZR types. The assays, in optimized formats, readily detected these plant hormones at concentration levels of 2–5 ng/ml, and showed high specificity for selected cytokinins. Certain assay parameters (e.g. the type of tracer enzyme, incubation and preincubation time, etc.) had a strong influence on detection sensitivity. Nonetheless, the assays appear robust showing tolerance to pH and to several water-miscible organic solvents. The described ELISA systems were sensitive enough to detect endogenous hormone levels in crude plant extracts without intense purification. In vitro selected transgenic tobacco and tomato lines showing tolerance to several stress factors proved to have higher levels of cytokinins than the corresponding control plants indicating that the developed immunoassay is suitable for the determination of stress resistance of plants by monitoring their cytokinin content.
immunoassay, ELISA, isopentenyl adenosine (IPA), isopentenyl adenine (2-iP), trans-zeatin riboside (ZR), trans-zeatin (Z), transgenic tobacco and tomato
Structure type: monomer
Location inside paper: abstract
Compound class: glycoside
Contained glycoepitopes: IEDB_149136
Methods: DNA techniques, ELISA, biological assays, extraction, centrifugation, immunological studies
Related record ID(s): 68083
NCBI Taxonomy refs (TaxIDs): 4097,
4081
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There is only one chemically distinct structure:
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Székács A, Hegedűs G, Tóbiás I, Pogány M, Barna B
Immunoassays for plant cytokinins as tools for the assessment of environmental stress and disease resistance
Analytica Chimica Acta 421(2) (2000)
135-146
|
Subst-(4-9)-A
Subst = (E)-2-methylbut-2-ene-1,4-diol = SMILES O{4}C/C=C({1}CO)\C |
Show graphically |
Nicotiana tabacum (CaMVp35S ipt mutant)
(Ancestor NCBI TaxID 4097,
species name lookup)
Nicotiana tabacum cv. Petit Havana
(Ancestor NCBI TaxID 4097,
species name lookup)
Nicotiana tabacum cv. Samsun
(Ancestor NCBI TaxID 4097,
species name lookup)
Lycopersicon esculentum cv. Linia XXIV
(later renamed to: Solanum lycopersicum cv. Linia XXIV)
(Ancestor NCBI TaxID 4081,
species name lookup)
Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Publication DOI: 10.1016/S0003-2670(00)01046-1Journal NLM ID: 0370534Correspondence: aszek
nki.hu
Institutions: Plant Protection Institute, Hungarian Academy of Sciences, Budapest, Hungary
The level of cytokinin type hormones present in plant tissues, such as N6-(2-isopentenyl)-adenosine (IPA), N6-(2-isopentenyl)-adenosine (2-iP), trans-zeatin riboside (ZR) and trans-zeatin (Z) is a good indicator of the resistance of plants to abiotic environmental stresses and to necrotic pathogens. Hapten-homologous and hapten-heterologous competitive indirect enzyme-linked immunosorbent assays (ELISAs) were developed, allowing the use of minute amounts of plant extracts for cytokinin analysis. These assays were used for the detection of members of the cytokinin plant hormone family including IPA and ZR types. The assays, in optimized formats, readily detected these plant hormones at concentration levels of 2–5 ng/ml, and showed high specificity for selected cytokinins. Certain assay parameters (e.g. the type of tracer enzyme, incubation and preincubation time, etc.) had a strong influence on detection sensitivity. Nonetheless, the assays appear robust showing tolerance to pH and to several water-miscible organic solvents. The described ELISA systems were sensitive enough to detect endogenous hormone levels in crude plant extracts without intense purification. In vitro selected transgenic tobacco and tomato lines showing tolerance to several stress factors proved to have higher levels of cytokinins than the corresponding control plants indicating that the developed immunoassay is suitable for the determination of stress resistance of plants by monitoring their cytokinin content.
immunoassay, ELISA, isopentenyl adenosine (IPA), isopentenyl adenine (2-iP), trans-zeatin riboside (ZR), trans-zeatin (Z), transgenic tobacco and tomato
Structure type: monomer
Location inside paper: abstract
Trivial name: zeatin riboside
Compound class: glycoside
Contained glycoepitopes: IEDB_140947
Methods: DNA techniques, ELISA, biological assays, extraction, centrifugation, immunological studies
Related record ID(s): 68082
NCBI Taxonomy refs (TaxIDs): 4097,
4081
Show glycosyltransferases
There is only one chemically distinct structure:
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