Taxonomic group: plant / Streptophyta
(Phylum: Streptophyta)
Organ / tissue: cell culture
NCBI PubMed ID: 10778921Publication DOI: 10.1016/s0378-4347(99)00473-9Journal NLM ID: 9421796Publisher: Amsterdam, Netherlands: Elsevier
Institutions: Department of Pharmacognosy, Gifu Pharmaceutical University, Gifu, Japan, Laboratory of Medicinal Plant Garden, School of Pharmaceutical Sciences, Nagasaki University, Nagasaki, Japan, Laboratory of Molecular & Cellular Biology of Totipotency, Division of Integrated Life Sciences, Graduate School of Biostudies, Kyoto University, Kyoto, Japan
A high-performance liquid chromatography (HPLC) analysis system based on a water-acetonitrile gradient program was established for simultaneous quantification of shikimate-derived secondary metabolites in cultured cells of Lithospermum erythrorhizon. The cells cultured in pigment production medium (M-9) are capable of producing five highly hydrophilic compounds such as p-hydroxybenzoic acid-O-glucoside and lithospermic acid B, as well as eleven lipophilic compounds including echinofuran B and acetylshikonin. In addition to the wide polarities of those compounds, many of them are unstable under light, dryness, oxygen and heating. Thus, a new extraction procedure for all these compounds was also established by use of ultrasonication under ice-water chilling with MeOH as the solvent. This procedure was applied to the quantitative analyses of these compounds in cell cultures and hairy root cultures of Lithospermum, and in the intact plants as well.
Lithospermum erythrorhizon; p-hydroxybenzoic acid-O-glucoside; shikonin; caffeic acid; echinofuran
Structure type: monomer
Location inside paper: p. 4, Fig. 1
Compound class: bDGlcp(1-7)xX4HOBz
Contained glycoepitopes: IEDB_142488,IEDB_146664,IEDB_983931,SB_192
Methods: HPLC, extraction, centrifugation
NCBI Taxonomy refs (TaxIDs): 34254
Show glycosyltransferases
There is only one chemically distinct structure:
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