Ascochyta caulina (P. Karst) v.d. Aa and v. Kest is a plant pathogenic fungus which is specific to Chenopodium album L. It has been suggested as a potential mycoherbicide to this weed, which is important and wide spread in arable crops throughout Europe. To investigate its potential as a biocontrol agent, the fungus has been tested in glasshouse and field experiments. Formulations containing different combinations of A. caulina conidia, the phytotoxins from the fungus and low doses of herbicides have been tested. Significant improvement in the efficacy of the fungus was achieved in glasshouse trials with an aqueous formulation containing PVA (0.1% v/v), Psyllium (0.4% w/v), Sylgard 309 (0.1% v/v), nutrients and conidia (5 x 10(6)/ml). The extracellular, hydrophilic phytotoxins produced by A. caulina were purified and their structures determined. The main toxin, named ascaulitoxin, was characterised as the N-2-β-D-glucopyranoside of the unusual bis-aminoacid 2,4,7-triamino-5-hydroxyoctandioic acid. Two other toxins proved to be trans-4-amino-D-proline and the aglycone of ascaulitoxin. These toxins have shown promising herbicidal properties. Field trials have investigated the performance of A. caulina conidia applied at different developmental stages of C. album either as a single treatment or combined with sub-lethal doses of herbicides or with the fungal phytotoxins. With the available formulation, favourable weather conditions are needed to obtain infection in the field. The efficacy of the strain of A. caulina used so far has proved to be inadequate to justify its development as a bioherbicide. This is probably due to its low virulence
Publication DOI: 10.1023/A:1011425826359Journal NLM ID: 9892014Publisher: Dordrecht, Netherlands: Kluwer Academic Publishers / Springer
Correspondence: jan.netland
planteforsk.no
Institutions: Norwegian Crop Research Institute, the Plant Protection Centre, Ås, Norway, Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol, UK, Istituto Tossine e Micotossine da Parassiti Vegetali, Consiglio Nazionale delle Ricerche, Bari, Italy, Dipartimento di Scienze Chimico-Agrarie, Università di Napoli Federico II, Portici, Italy, Humboldt University Fachgebiet Ackerbausysteme, Berlin, Germany, Plant Research International, Wageningen, The Netherlands
Methods: 13C NMR, 1H NMR, gel filtration, FAB-MS, biological assays, UV, HR-EI-MS
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