A polysaccharide containing D-Gal, D-GalNAc, 3-(L-2-acetoxypropionamido)-3,6-dideoxy-D-galactose (approximately 80%) and 3-(L-2-hydroxypropionamido)-3,6-dideoxy-D-galactose (approximately 20%) was isolated by mild acid hydrolysis, followed by gel-permeation chromatography, from the phenol-soluble lipopolysaccharide (phenol/water extracted) derived from Acinetobacter strain 94. The polysaccharide, characterised by means of monosaccharide analyses, partial acid hydrolysis, and NMR studies, consisted of a branched tetrasaccharide repeating unit, as depicted below, in which Fucp3Nacyl represents 3-(L-2-hydroxypropionamido)-3,6-dideoxy-D-galactose, in which approximately 80% of the acyl residues are O-acetylated. These Fucp3N derivatives and an O-acetylated acyl group are therefore constituents of bacterial LPS, but to our knowledge are not present in any other natural carbohydrates. [sturcture: see text]

Lipopolysaccharide, LPS, DNA, strain, structural, polysaccharide, O-antigen, group, O-antigenic, O-antigenic polysaccharide, acid, Acinetobacter, 6-dideoxy-D-galactose, polysaccharides, structural studies, 3-amino-3, 2-acetoxypropionyl, amide-linked, phenol soluble

• Compound ID: 2086
  

  S-Lac2(80%)Ac-(1-3)-a-D-Fucp3N-(1-4)-b-D-GalpNAc-(1-4)-+ | -3)-a-D-Galp-(1-3)-b-D-GalpNAc-(1-

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  Structure type: polymer chemical repeating unit
  Compound class: O-polysaccharide, O-antigen
  
   
  
  Acinetobacter sp. 94 (DNA group 11)
  CSDB ID 492 (all data & tools)