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1. (Article ID: 6943)
 
Haido RM, Silva MH, Ejzemberg R, Leitao EA, Hearn VM, Evans EG, Barreto Bergter E
Analysis of peptidogalactomannans from the mycelial surface of Aspergillus fumigatus
Medical Mycology 36 (1998) 313-321
 

Peptidogalactomannans (pGMs) from mycelium of two strains of Aspergillus fumigatus were fractionated by Cetavlon precipitation and size exclusion chromatography and theircarbohydrate structures analysed using methylation-fragmentation analysis, partial acetolysis and 13C-nuclear magnetic resonance spectroscopy. The most significant difference between the pGMs of the two strains was the degree of branching and the proportion of non-reducing ends of α-D-Manp) and β-D-Galf units. Methylation data showed that the pGM from AF 2109 contained α-D-Man p) and β-D-Galf non-reducing end units in a proportion of 3:1 while, in contrast, the proportion of these structures in pGM from AF 2140 was 7:1, resulting in a highly branched structure. The immunoreactivity of the pGM fractions was tested by indirect immunofluorescence. The fractions were also tested in an ELISA system with rabbit antiserum raised to whole cells of A. fumigatus NCPF 2140 and with serum from patients with either proven aspergilloma or ABPA. The carbohydrate moiety of the pGM appears to be responsible for the antigenicity. Periodate treatment, partial acid hydrolysis and β-elimination removed most of the antibody binding capacity.

Aspergillus fumigatus, 13C-nuclear magnetic resonance, glycoprotein immunoreactivity, peptidogalactomannans

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2. (Article ID: 7242)
 
Boas MH, Egge H, Pohlentz G, Hartmann R, Bergter EB
Structural determination of N-2'-hydroxyoctadecenoyl-1-O-beta-D-glucopyranosyl-9-methyl-4,8-sphingadienine from species of Aspergillus
Chemistry and Physics of Lipids 70(1) (1994) 11-19
 

Ceramide monohexosides from Aspergillus fumigatus 2140 and 2109 strains and Aspergillus versicolor 550 strain, obtained by silica gel 60, and Iatrobeads chromatography were analysed using high-resolution 1D-, 2D-1H-NMR and 13C-NMR spectroscopy and fast atom bombardment mass spectrometry (FAB-MS). The ceramide monohexoside fraction (CMH) from A. fumigatus 2140 and A. versicolor 550 was identified as glucosylceramide, whereas glucose and galactose were present at a ratio of 1:1 in the CMH of A. fumigatus 2109. The major glycosphingolipid has a particular ceramide composition consisting of 9-methyl-4,8-sphingadienine linked to a 2-hydroxyoctadec-3-enoic acid. Although the structures presently described are similar to those of monohexosylceramides from other fungi, including edible ones, this is the first report on their occurrence in species pathogenic in humans.

FAB-MS, 13C-NMR spectroscopy, galactosylceramide, Aspergillus fumigatus, glucosylceramide, Aspergillus versicolor, 1H-NMR spectroscopy

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