Vibrio parahaemolyticus strain KX-V212 of a novel serotype, which does not belong to any of the known 13 O-serotypes of this vibrio, was isolated from a patient. Its O-antigen harbors a unique strain-specific O-antigenic factor(s), in addition to that shared by the O-antigen of V. parahaemolyticus serotype O2. A carbohydrate backbone nonasaccharide was isolated from the lipopolysaccharide (LPS) of strain KX-V212 by dephosphorylation, reduction and deacylation and found to consist of one residue each of -glucose, -galactose, -GlcN, 3-deoxy--manno-oct-2-ulosonic acid (Kdo) and 5-acetamido-7-(N-acetyl--alanyl)amino-3,5,7,9-tetradeoxy--glycero--galacto-non-2-ulosonic acid (Non5Ac7Ala), and two residues each of -GlcA and -glycero--manno-heptose (-Hep). Analysis of the isolated and deacylated lipid A showed that this oligosaccharide was an artifact resulting from a loss of one GlcN residue from the lipid A backbone. Therefore, the carbohydrate backbone of the LPS is a decasaccharide having the structure shown below. The initial LPS contains also -GalA and phosphoethanolamine at unknown positions. Both similarity and differences are observed between the LPS of V. parahaemolyticus serotype O2 and strain KX-V212.
Lipopolysaccharide, lipopolysaccharides, structure, strain, structural, characterization, carbohydrate, acid, serological, backbone, Vibrio, Vibrio parahaemolyticus
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