The exopolysaccharide, Botryosphaeran, produced by the ligninolytic, ascomycetcous fungus Botryosphaeria sp., was isolated from the extracellular fluid by precipitation with ethanol, and purified by gel permeation chromatography to yield a carbohydrate-rich fraction (96%) composed mainly of glucose (98%). Infra-red and C-13 NMR spectroscopy showed that all the glucosidic linkages were in the β-configuration. Data from methylation analysis and Smith degradation indicated that Botryosphaeran was a (1→3)-β-(D)-glucan with approx 22% side branching at C-6. The products obtained from partial acid hydrolysis demonstrated that the side branches consisted of single (1→6)-β-linked glucosyl, and (1→6)-β-linked gentiobiosyl residues.

exopolysaccharide, (1 -> 3;1 -> 6)-beta-glucan, Botryosphaeran, Botyrosphaeria sp., Ascomyceteous fungus

Publication DOI: 10.1016/S0008-6215(03)00240-4
Journal NLM ID: 0043535
Publisher: Elsevier
Correspondence: corradiprudente.unesp.br
Institutions: Departamento de Bioquı́mica, Universidade Estadual de Londrina, CCE, CEP 86051-990 Londrina, PR, Brazil, Departamento de Bioquı́mica, Universidade do Oeste Paulista, CEP 19050-900 Presidente Prudente, SP, Brazil, Departamento de Fı́sica, Quı́mica e Biologia, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista, CEP 19060-900 Presidente Prudente, SP, Brazil
Methods: 13C NMR, methylation, partial acid hydrolysis, GC-MS, sugar analysis, Smith degradation, FTIR, GPC, enzymatic digestion, HPAEC-PAD, acetylation
 

• Compound ID: 4415
  

  -3)-b-D-Glcp-(1-

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  Structure type: homopolymer
  Trivial name: glucan, β-1,3-glucan, curdlan, curdlan-type polysaccharide 13140, paramylon, curdlan, laminarin, β-glucan, curdlan, β-(1,3)-glucan, β-(1,3)-glucan, curdlan, curdlan, β-1,3-glucan, paramylon, reserve polysaccharide, b-glucan, β-1,3-D-glucan, laminaran, botryosphaeran, laminaran type β-D-glucan, latiglucan I, pachymaran, Curdlan, zymosan A, β-glucan, curdlan, laminarin, zymosan, zymosan, glucan particles, zymosan, β-(1-3)-glucan, β-(1,3)-glucan, β-(1,3)glucan, pachymaran, D-glucan (DPn)540, pachyman, laminaran, curdlan, zymosan, zymosan, β-(1,3)-glucan, zymosan A, zymosan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, β-1,3-glucan, curdlan, pachyman, β-(1,3)-glucan, curdlan, callose, a water-insoluble β-(1→3)-glucan, fermentum β-polysaccharide, water-insoluble glucan, alkali-soluble β-glucan (PeA3), alkali-soluble polysaccharide (PCAP), callose, laminarin
  Compound class: EPS, O-polysaccharide, cell wall polysaccharide, lipophosphoglycan, glycoprotein, LPG, glucan, polysaccharide, glycoside, β-glucan, β3-glucan, cell wall glucan
  Reference(s) to other database(s): GTC:G51056AN, GlycomeDB:157, CCSD:50049, CBank-STR:4225, CA-RN: 51052-65-4, GenDB:FJ3380871.1
  
   
  
  Botryosphaeria sp. MAMB-5
  CSDB ID 41370 (all data & tools)
• Compound ID: 17895
  

  b-D-Glcp-(1-6)-+ | -3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-

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  Structure type: polymer chemical repeating unit ; n=5
  Trivial name: Botryosphaeran
  Compound class: EPS
  Reference(s) to other database(s): GTC:G47692IC
  
   
  
  Botryosphaeria sp. MAMB-5
  CSDB ID 41369 (all data & tools)
• Compound ID: 17896
  

  b-D-Glcp-(1-6)-b-D-Glcp-(1-6)-+ | -3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-

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  Structure type: polymer chemical repeating unit ; n=5
  Trivial name: Botryosphaeran
  Compound class: EPS
  Reference(s) to other database(s): GTC:G62581DR
  
   
  
  Botryosphaeria sp. MAMB-5
  CSDB ID 41385 (all data & tools)