Four exopolysaccharides (EPS) obtained from Botryosphaeria rhodina strains isolated from rotting tropical fruit (graviola, mango, pinha, and orange) grown on sucrose were purified on Sepharose CL-4B. Total acid hydrolysis of each EPS yielded only glucose. Data from methylation analysis and (13)C NMR spectroscopy indicated that the EPS from the graviola isolate consisted of a main chain of glucopyranosyl (1→3) linkages substituted at O-6 as shown in the putative structure below: [carbohydrate structure: see text]. The EPS of the other fungal isolates consisted of a linear chain of (1→6)-linked glucopyranosyl residues of the following structure: [carbohydrate structure: see text]. FTIR spectra showed one band at 891 cm(-1), and (13)C NMR spectroscopy showed that all glucosidic linkages were of the β-configuration. Dye-inclusion studies with Congo Red indicated that each EPS existed in a triple-helix conformational state. β-(1→6)-d-Glucans produced as exocellular polysaccharides by fungi are uncommon.

exopolysaccharides, Botryosphaeria rhodina isolates, β(1-6)-d-Glucans, β(1-3;1-6)-d-Glucans, Triple-helix conformation

NCBI PubMed ID: 18639868
Publication DOI: 10.1016/j.carres.2008.06.013
Journal NLM ID: 0043535
Publisher: Elsevier
Correspondence: corradifct.unesp.br (M. de Lourdes Corradi da Silva)
Institutions: Departamento de Física, Química e Biologia, Faculdade de Ciências e Tecnologia, Universidade Estadual Paulista, Presidente Prudente, São Paulo, Brazil, Departamento de Bioquímica e Microbiologia, Instituto de Biociências, Universidade Estadual Paulista, Rio Claro, São Paulo, Brazil, Universidad de Castilla—La Mancha, Instituto de Regional Investigación Científica Aplicada (IRICA), Real, Spain, Departamento de Bioquímica e Biotecnologia—CCE, Universidade Estadual de Londrina, Londrina, Paraná, Brazil, Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, Curitiba, Paraná, Brazil, Departamento de Química e Ciências Ambientais, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista, São José do Rio Preto, São Paulo, Brazil
Methods: 13C NMR, methylation, GC-MS, sugar analysis, conformation analysis, acid hydrolysis, FTIR, GPC, HPAEC-PAD, dialysis
 

• Compound ID: 18115
  

  b-D-Glcp-(1-6)-+ | -3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-3)-b-D-Glcp-(1-

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  Structure type: structural motif or average structure
  Trivial name: pleuran, lentinan, scleroglucan, schizophyllan, grifolan
  Compound class: EPS, glucan, polysaccharide
  Reference(s) to other database(s): GTC:G06474SE
  
   
  
  Botryosphaeria rhodina MMGR
  CSDB ID 41760 (all data & tools)
• Compound ID: 6167
  

  -6)-b-D-Glcp-(1-

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  Structure type: homopolymer
  Trivial name: pustulan, β-1,6-glucan, β-1,6-D-glucan, β(1-6)-D-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-(1,6)-glucan, lasiodiplodan, β-(1,6)-glucan, β-(1,6)-glucan, lasiodiplodan, pustulan, β-1,6-glucan, β-(1,6)-glucan, pustulan, β-(1→6)-glucan PCPS, water-soluble glucan (PS-I)
  Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glycoprotein, glucan, polysaccharide, cell wall glucoprotein
  Reference(s) to other database(s): GTC:G26777BZ, GlycomeDB:863, CCSD:50854, CBank-STR:4234
  
   
  
  Botryosphaeria rhodina MMMFR, Botryosphaeria rhodina MMPI, Botryosphaeria rhodina MMLR
  CSDB ID 41968 (all data & tools)