CSDB: Search results

The water-soluble crude polysaccharides were obtained from the fruiting bodies of cultured Cordyceps militaris by hot water extraction followed by ethanol precipitation. The polysaccharides were successively purified by chromatography on DEAE–cellulose-52 and Sephacryl S-100 HR columns, giving main three polysaccharide fractions termed P50-1, P70-1, and P70-2. Structural features of P70-1 were investigated by a combination of chemical and instrumental analysis, such as partial acid hydrolysis, methylation analysis, periodate oxidation – Smith degradation, GC–MS, 13C NMR, HPAEC-PAD, and FT-IR. The results indicated that P70-1 has a backbone of (1→6)-linked β-D-mannopyranosyl residues, which occasionally branches at O-3. The branches were mainly composed of (1→4)-linked α-d-glucopyranosyl and (1→6)-linked β-D-galactopyranosyl residues, and terminated with β-D-galactopyranosyl residues and α-d-glucopyranosyl residues. In the in vitro antioxidant assay, P70-1 was found to possess hydroxyl radical-scavenging activity with an IC50 value of 0.548 mg/ml.

polysaccharide structure, Antioxidant, fruiting bodies, Cultured Cordyceps militaris, HPAEC-PAD analysis

Publication DOI: 10.1016/j.carbpol.2007.05.005
Journal NLM ID: 8307156
Publisher: Elsevier
Correspondence: workspacerongminyu99

hotmail.com (Rongmin Yu)
Institutions: Jinan University College of Pharmacy, Guangzhou 510632, China, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310038, China
Methods: 13C NMR, methylation, periodate oxidation, partial acid hydrolysis, GC-MS, ESI-MS, acid hydrolysis, GC, Smith degradation, FTIR, composition analysis, GPC, HPAEC-PAD, extraction, statistical analysis, antioxidant activities