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1. (Article ID: 7123)
 
Prieto A, Leal JA, Giménez-Abián MI, Canales A, Jiménez-Barbero J, Bernabé M
Isolation and structural determination of a unique polysaccharide containing mannofuranose from the cell wall of the fungus Acrospermum compressum
Glycoconjugate Journal 24(8) (2007) 421-428
 

The alkali-extractable and water-soluble fungal polysaccharide F1SS isolated from the cell wall of Acrospermum compressum has been studied by methylation analyses, reductive cleavage and 1D- and 2D-NMR spectroscopy. The polysaccharide consists of a regular disaccharide repeating unit with the structure: [structure: see text]. The mannan core was obtained by mild hydrolysis of the polysaccharide F1SS and its structure was deduced to be composed of a skeleton of α-(1→6)-mannopyranan, with around 1 out of 11 residues substituted at position 2 by short chains (one to six units) of 2-substituted mannopyranoses. DOSY experiments provided molecular sizes of 60 kDa and 2.5 kDa for the polysaccharide F1SS and the Mannan core, respectively. This is the first report of a fungal mannofuranose-containing cell wall polysaccharide.

NMR spectroscopy, cell wall polysaccharide, fungi, Acrospermaceae, mannofuranose

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