Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 9203334Journal NLM ID: 0043535Publisher: Elsevier
Institutions: Institute of Chemistry and Biochemistry ''G. Ronzoni'', I-20133 Milan, Italy
13C NMR relaxation measurements at three different magnetic field strengths have been used to analyse the motional properties of a low molecular weight K5 polysaccharide (∆ UA-[→4)-β-D-GlcNAc(1→4)-β-D-GlcA(1→]n-GlcNAcred) from E. coli. Two-dimensional double INEPT spectra with suppression of cross-correlation effects between dipolar and chemical shift anisotropy relaxation mechanisms were collected in order to determine carbon longitudinal and transverse relaxation times. The values of the overall correlation time and the rate of internal motions were obtained using the model free spectral densities. The data indicate that the overall motion of the molecule is non-isotropic and can be approximated with the symmetric top model with an axial ratio of approximately 22. The magnitude of the generalized order parameters (S2 approximately 0.8) and the internal motion correlation time (tau e approximately 30 ps) differ from those found for iduronyl-containing glycosaminoglycans and suggest that the internal motions in K5 polysaccharide are more limited.
capsular polysaccharide, molecular dynamics, NMR relaxation, glycosaminoglycan, heparin
Structure type: polymer chemical repeating unit
Location inside paper: p.69-76
Trivial name: glycosaminoglycan
Compound class: CPS
Contained glycoepitopes: IEDB_115136,IEDB_135813,IEDB_137340,IEDB_140630,IEDB_141807,IEDB_151531,IEDB_153764,IEDB_423153
Methods: MD simulations, NMR relaxation
3D data: 3D data
NCBI Taxonomy refs (TaxIDs): 562Reference(s) to other database(s): GTC:G60502DO, GlycomeDB:
27096
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There is only one chemically distinct structure:
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