Taxonomic group: bacteria / Proteobacteria
(Phylum: Proteobacteria)
Associated disease: infection due to Escherichia coli [ICD11:
XN6P4 
]
NCBI PubMed ID: 10762260Publication DOI: 10.1128/JB.182.9.2567-2573.2000Journal NLM ID: 2985120RPublisher: American Society for Microbiology
Correspondence: j45811a
nucc.cc.nagoya-u.ac.jp
Institutions: Unit of Biosystems, School of Informatics and Sciences, Nagoya University, Nagoya, Aichi 464-8601, Department of Food Hygienic Chemistry, Faculty of Home Economics, Kyushu Women's University, Kitakyushu City, Fukuoka 807-8586,2Japan.
wbdA is a mannosyltransferase gene that is involved in synthesis of the Escherichia coli O9a polysaccharide, a mannose homopolymer with a repeating unit of 2-αMan-1,2-αMan-1,3-αMan-1, 3-αMan-1. The equivalent structural O polysaccharide in the E. coli O9 and Klebsiella O3 strains is 2-αMan-1,2-αMan-1, 2-αMan-1,3-αMan-1,3-αMan-1, with an excess of one mannose in the 1,2 linkage. We have cloned wbdA genes from these O9 and O3 strains and shown by genetic and functional studies that wbdA is the only gene determining the O-polysaccharide structure of O9 or O9a. Based on functional analysis of chimeric genes and site-directed mutagenesis, we showed that a single amino acid substitution, C55R, in WbdA of E. coli O9 converts the O9 polysaccharide into O9a. DNA sequencing revealed the substitution to be conserved in other E. coli O9a strains. The reverse substitution, R55C, in WbdA of E. coli O9a resulted in lipopolysaccharide synthesis showing no ladder profile instead of the conversion of O9a to O9. This suggests that more than one amino acid substitution in WbdA is required for conversion from O9a to O9.
polysaccharide, Escherichia, Escherichia coli, amino acid, O-serotype
Structure type: polymer chemical repeating unit
Location inside paper: p.2567
Trivial name: mannose homopolysaccharide
Compound class: O-polysaccharide, O-antigen
Contained glycoepitopes: IEDB_115576,IEDB_130701,IEDB_136104,IEDB_140116,IEDB_141111,IEDB_141795,IEDB_141830,IEDB_143632,IEDB_144983,IEDB_152206,IEDB_153756,IEDB_164174,IEDB_164175,IEDB_164176,IEDB_164480,IEDB_174840,IEDB_241100,IEDB_76933,IEDB_983930,SB_136,SB_196,SB_197,SB_44,SB_67,SB_72
Methods: 1H NMR
Biosynthesis and genetic data: biochemical data, genetic data
Comments, role: published polymerization frame was shifted for conformity with other records.
Related record ID(s): 883, 5762, 7236, 10527, 20624, 25215
NCBI Taxonomy refs (TaxIDs): 1010797Reference(s) to other database(s): GTC:G87424QV, GlycomeDB:
668, CCSD:
47538
Show glycosyltransferases
There is only one chemically distinct structure:
Found 
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