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1. (Article ID: 7199)
Choudhury AR, Saluja P, Prasad GS
Pullulan production by an osmotolerant Aureobasidium pullulans RBF-4A3 isolated from flowers of Caesulia axillaris
Carbohydrate Polymers 83(4) (2011)
1547-1552
Phenotypic and molecular characterization of five yeast-like fungal isolates from flowers of wild plants showed that they are related to Aureobasidium pullulans. Compared to other isolates, an osmotolerant and non-pigmented isolate A. pullulans RBF-4A3 produced 26.35 g l-1 of melanin-free exopolysaccharide (EPS) in 96 h at 30 °C in 5% glucose containing medium. At higher concentrations of glucose (7.5-25% (w/v)), the EPS produced by this organism increased from 34.68 to 66.79 g l-1 up to 15% (w/v) glucose, with a productivity of 16.69 g l-1 per day. Beyond 15% (w/v) glucose concentration, the EPS production decreased gradually to 43.29 g l-1 at 25% (w/v) glucose. Fourier-transform infrared (FTIR) spectroscopy confirmed that chemical structures of the exopolysaccharide produced by A. pullulans RBF-4A3 and standard pullulan were identical. This is the first report of pullulan production at 15% (w/v) concentration of glucose by an osmotolerant strain of A. pullulans.
exopolysaccharide, fermentation, pullulan, Aureobasidium pullulans, osmotolerant
Publication DOI: 10.1016/j.carbpol.2010.10.003Journal NLM ID: 8307156Publisher: Elsevier
Correspondence: prasad
imtech.res.in
Institutions: Biochemical Engineering Research and Process Development Centre (BERPDC), Institute of Microbial Technology (IMTECH), Council of Scientific and Industrial Research, Chandigarh, India, Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology (IMTECH), Council of Scientific and Industrial Research, Chandigarh, India
Methods: FTIR, cell growth, precipitation
The publication contains the following compound(s):
- Compound ID: 12430
Structure type: polymer chemical repeating unit
Trivial name: pullulan
Compound class: EPS, O-polysaccharide, cell wall polysaccharide, glucan, polysaccharide
Reference(s) to other database(s): GTC:G71532WE, CCSD:
45938, CBank-STR:4859, GenDB:KY767023; GenDB:KY767024
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