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1. (Article ID: 7240)
Rau U, Nguyen LA, Roeper H, Koch H, Lang S
Fed-batch bioreactor production of mannosylerythritol lipids secreted by Pseudozyma aphidis
Applied Microbiology and Biotechnology 68(5) (2005)
607-613
Two strains of Pseudozyma aphidis, DSM 70725 and DSM 14930, were used for the bioreactor production of mannosylerythritol lipids (MELs). Foam formation interfered substantially with the cultivation process. Soybean oil was simultaneously employed as both carbon source and anti-foam agent. Primary MEL formation occurred after nitrate limitation. After a first short time-period of nitrate limitation and further nitrate addition, MELs were secreted in spite of nitrate excess. The sedimentation of MEL-enriched beads indicated enhanced product formation. Maximum yield, productivity and yield coefficient of 165 g l(-1), 13.9 g l(-1) day(-1) and 0.92 g g(-1) were achieved using strain DSM 14930 with additional substrate-feeding (glucose, sodium nitrate, yeast extract) and a foam-controlled soybean oil supply.
mannosylerythritol
NCBI PubMed ID: 15729556Publication DOI: 10.1007/s00253-005-1906-5Journal NLM ID: 8406612Publisher: Springer
Correspondence: U.Rau
tu-bs.de
Institutions: Cargill R&D Europe, Vilvoorde, Belgium, Institute of Biochemistry and Biotechnology, Technical University Braunschweig, Brunswick, Germany, C12 P4 Khu tap the Kim Giang, Hanoi, Vietnam
Methods: HPLC, extraction, CC
The publication contains the following compound(s):
- Compound ID: 18411
Structure type: oligomer
Trivial name: mannosylerythritol, MEL, mannosylerythritol, mannosylerythritol lipid
Compound class: glycolipid
Reference(s) to other database(s): GenDB:AF038826
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