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1. (Article ID: 7267)
 
Han XQ, Wu XM, Chai XY, Chen D, Dai H, Dong HL, Ma ZZ, Gao XM, Tu PF
Isolation, characterization and immunological activity of a polysaccharide from the fruit bodies of an edible mushroom, Sarcodon aspratus (Berk.) S. Ito.
Food Research International 44(1) (2011) 489-493
 

A water-soluble polysaccharide (HCP) with a molecular mass of 6.7×105Da determined by high performance size-exclusion chromatography (HPSEC), was isolated from the fruit bodies of Sarcodon aspratus (Berk.) S. Ito., an edible mushroom. HCP was elucidated as a liner glucan with a backbone structure of (1→6)-linked-α-D-glucopyranosyl residues by interpretation of the composition analysis, methylation analysis, periodate oxidation experiment, FT-IR, and NMR spectroscopy. Immunological activity evaluation using H3-thymidine incorporation method revealed that HCP could significantly stimulate the proliferation of the cultured mice spleen lymphocyte in a dose-dependent manner. HCP might be a potential immunomodulator which can be used against pathogens and tumors in health-care food or medicine. This is the first report on the detailed structure elucidation of the polysaccharide from S. aspratus.

polysaccharide, glucan, immunological activity, Edible mushroom, Sarcodon aspratus (Berk.) S. Ito.

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