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Taxonomic group: protista / Euglenozoa (Phylum: Euglenozoa)
Host organism: Homo sapiens
Associated disease: infection due to Leishmania [ICD11: XN8JE

]

NCBI PubMed ID: 29491743
Publication DOI: 10.2174/1389202918666170815143331
Journal NLM ID: 100960527
Publisher: Saif Zone, Sharjah, U.A.E.: Bentham Science Publishers
Correspondence: vyacheslav.yurchenko

osu.cz
Institutions: Life Science Research Centre, Faculty of Science, University of Ostrava, 710 00 Ostrava, Czech Republic, Biology Centre, Institute of Parasitology, Czech Academy of Sciences, 370 05 České Budejovice (Budweis), Czech Republic, Centro de Pesquisas Rene Rachou/FIOCRUZ, Belo Horizonte, Minas Gerais, 30190-002, Brazil, Zoological Institute of the Russian Academy of Sciences, St. Petersburg199034, Russia, de Duve Institute, Universite Catholique de Louvain, 1200, Brussels, Belgium

Background: Leptomonas pyrrhocoris is a parasite of the firebug Pyrrhocoris apterus. This flagellate has been recently proposed as a model species for studying different aspects of the biology of monoxenous trypanosomatids, including host - parasite interactions. During its life cycle L. pyrrhocoris never tightly attaches to the epithelium of the insect gut. In contrast, its dixenous relatives (Leishmania spp.) establish a stable infection via attachment to the intestinal walls of their insect hosts. Material and methods: This process is mediated by chemical modifications of the cell surface lipophosphoglycans. In our study we tested whether the inability of L. pyrrhocoris to attach to the firebug's midgut is associated with the absence of these glycoconjugates. We also analyzed evolution of the proteins involved in proper lipophosphoglycan assembly, cell attachment and establishment of a stable infection in L. pyrrhocoris, L. seymouri, and Leishmania spp. Our comparative analysis demonstrated differences in SCG/L/R repertoire between the two parasite subgenera, Leishmania and Viannia, which may be related to distinct life strategies in various Leishmania spp. The genome of L. pyrrhocoris encodes 6 SCG genes, all of which are quite divergent from their orthologs in the genus Leishmania. Using direct probing with an antibody recognizing the β-Gal side chains of lipophosphoglycans, we confirmed that these structures are not synthesized in L. pyrrhocoris. Conclusion: We conclude that either the SCG enzymes are not active in this species (similarly to SCG5/7 in L. major), or they possess a different biochemical activity.

Host-parasite interaction, Insect gut's attachment, L.pyrrhocoris, LPG, Monoxenous trypanosomatids, SCG enzymes

Structure type: oligomer
Location inside paper: p.151
Compound class: LPG
Contained glycoepitopes: IEDB_130701,IEDB_134623,IEDB_134624,IEDB_136044,IEDB_136095,IEDB_136100,IEDB_136906,IEDB_137472,IEDB_141794,IEDB_141807,IEDB_142349,IEDB_142350,IEDB_142488,IEDB_144983,IEDB_144993,IEDB_144996,IEDB_144998,IEDB_145002,IEDB_146664,IEDB_151528,IEDB_151531,IEDB_152206,IEDB_164174,IEDB_190606,IEDB_433717,IEDB_474450,IEDB_983930,IEDB_983931,SB_163,SB_165,SB_166,SB_187,SB_192,SB_195,SB_197,SB_44,SB_67,SB_7,SB_72,SB_88

Methods: Western blotting, phylogenetic analysis, TEM, genome analysis, light electron microscopy

NCBI Taxonomy refs (TaxIDs): 5658
Show glycosyltransferases

There is only one chemically distinct structure:

[1*]OCC(COP(=O)(O)O[C@H]1[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O[C@H]1O[C@H](CO)[C@@H](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](O[C@H]3O[C@H](COP(=O)(O)O[C@H]4O[C@H](CO)[C@@H](O)[C@H](O)[C@H]4O)[C@@H](O)[C@H](O[C@@H]4O[C@@H]([C@H](O)CO)[C@H](O[C@H]5O[C@H](CO[C@H]6O[C@H](COP(=O)(O)O[C@H]7O[C@H](CO)[C@@H](O[C@@H]8O[C@H](COP(=O)(O)O[C@H]9O[C@H](CO)[C@@H](O[C@@H]%10O[C@H](COP(=O)(O)O[C@H]%11O[C@H](CO)[C@@H](O[C@@H]%12O[C@H](CO)[C@H](O)[C@H](O)[C@H]%12O)[C@H](O)[C@@H]%11O)[C@H](O)[C@H](O)[C@H]%10O)[C@H](O)[C@@H]9O)[C@H](O)[C@H](O)[C@H]8O)[C@H](O)[C@@H]7O)[C@H](O)[C@H](O)[C@H]6O)[C@H](O)[C@H](O)[C@H]5O)[C@H]4O)[C@@H]3O)[C@@H]2O)[C@H](O)[C@H]1N)O[2*]

SVG MW SMILES 3D mol Ionize

R1 = ALK
R2 = LIP
Warning = sub-repeat ending at xXP?: unknown unit count was assumed to be 3